PMID- 15010371
OWN - NLM
STAT- MEDLINE
DCOM- 20040827
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 104
IP  - 1
DP  - 2004 Jul 1
TI  - Human B cells that hyperexpress a triad of costimulatory molecules via 
      avipox-vector infection: an alternative source of efficient antigen-presenting 
      cells.
PG  - 192-9
AB  - Dendritic cells (DCs) are the most potent of the antigen-presenting cells (APCs). 
      Preparation of sufficient numbers of mature DCs, however, is both costly and 
      time-consuming. We have examined here the possibility of using an alternative 
      source of APCs that would be easier to obtain, would not require extensive 
      culture, and thus would be more applicable to human immunotherapy protocols. We 
      show here that freshly isolated human B cells can be efficiently infected by a 
      replication-defective fowlpox recombinant vector, designated rF-TRICOM (TRIad of 
      COstimulatory Molecules), to markedly increase surface expression of the human 
      costimulatory molecule B7-1 and moderately increase expression of intercellular 
      adhesion molecule-1 (ICAM-1) and leukocyte function-associated antigen-3 (LFA-3). 
      Peptide-pulsed rF-TRICOM-infected B cells were highly efficient in activating 
      antigen-specific human T cells and shown to be superior to the use of CD40L in 
      enhancing APC potency. Moreover, when infection of freshly isolated B cells with 
      rF-TRICOM was combined with CD40L, a still further marked enhancement of the 
      antigen-presenting potency was observed. Ex vivo-generated antigen-specific T 
      cells activated in this manner might be applied to experimental protocols or used 
      for adoptive transfer in immunotherapy protocols.
FAU - Palena, Claudia
AU  - Palena C
AD  - Laboratory of Tumor Immunology and Biology, Center for Cancer Research, National 
      Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
FAU - Zhu, MingZhu
AU  - Zhu M
FAU - Schlom, Jeffrey
AU  - Schlom J
FAU - Tsang, Kwong-Yok
AU  - Tsang KY
LA  - eng
PT  - Journal Article
DEP - 20040309
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (B7-1 Antigen)
RN  - 0 (CD58 Antigens)
RN  - 0 (Carcinoembryonic Antigen)
RN  - 0 (Peptides)
RN  - 0 (Viral Proteins)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 147205-72-9 (CD40 Ligand)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 3.4.21.77 (Prostate-Specific Antigen)
SB  - IM
MH  - Antigen-Presenting Cells/*immunology/metabolism/virology
MH  - B-Lymphocytes/*immunology/metabolism/*virology
MH  - B7-1 Antigen/*biosynthesis/immunology
MH  - CD40 Ligand/immunology
MH  - CD58 Antigens/*biosynthesis/immunology
MH  - CD8-Positive T-Lymphocytes/immunology/metabolism
MH  - Carcinoembryonic Antigen/immunology
MH  - Cell Line
MH  - Fowlpox virus/genetics/*immunology
MH  - Gene Expression
MH  - Genetic Vectors/genetics
MH  - Herpesvirus 4, Human/genetics/immunology
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/*biosynthesis/immunology
MH  - Interferon-gamma/biosynthesis
MH  - Leukocytes, Mononuclear/cytology
MH  - Lymphocyte Activation/immunology
MH  - Papillomaviridae/immunology
MH  - Peptides/immunology/metabolism
MH  - Phenotype
MH  - Prostate-Specific Antigen/immunology
MH  - Viral Proteins/chemistry/metabolism
EDAT- 2004/03/11 05:00
MHDA- 2004/08/28 05:00
CRDT- 2004/03/11 05:00
PHST- 2004/03/11 05:00 [pubmed]
PHST- 2004/08/28 05:00 [medline]
PHST- 2004/03/11 05:00 [entrez]
AID - S0006-4971(20)54525-4 [pii]
AID - 10.1182/blood-2003-09-3211 [doi]
PST - ppublish
SO  - Blood. 2004 Jul 1;104(1):192-9. doi: 10.1182/blood-2003-09-3211. Epub 2004 Mar 9.