PMID- 15010867
OWN - NLM
STAT- MEDLINE
DCOM- 20041129
LR  - 20061115
IS  - 1021-335X (Print)
IS  - 1021-335X (Linking)
VI  - 11
IP  - 4
DP  - 2004 Apr
TI  - Fluorescence in situ hybridization detects frequent chromosome 9 deletions and 
      aneuploidy in histologically normal urothelium of bladder cancer patients.
PG  - 745-51
AB  - Investigation of early urothelial lesions is essential to gain insight into the 
      development of bladder cancer. In order to evaluate if deletions of chromosome 
      arms 9p and 9q are preceding histomorphological changes in the urothelium, 
      histologically normal urothelial samples of patients with previous and/or 
      simultaneous (pre)malignant urothelial lesions were investigated. Dual colour 
      fluorescence in situ hybridization (FISH) was performed on 96 histologically 
      inconspicuous urothelial biopsies from 41 patients. Centromeric probes for 
      chromosomes 9 and 17 were combined with gene locus specific probes for 9p21 
      (p16/CDKI2), 9q22 (FACC) and 17p (p53). Deletions of chromosome 9 (defined as 
      presence of only one copy of centromere 9 or fewer gene-specific than centromeric 
      signals in at least 40% of evaluated cells) were found in 21% of the samples 
      (18/87). Deletions of chromosome 9p were present in 16% of cases (14/89), whereas 
      deletions of chromosome 9q were encountered in 10% of specimens (9/93). A 
      hemizygous deletion of p53 was found only once. Polysomy in more than 10% of 
      cells was encountered in 16% of cases for both chromosome 9 and chromosome 17. 
      Non-diploidy (defined as polysomy of both chromosomes) was found in 6% of samples 
      (5/80). In summary, chromosome 9 deletions are frequently found in histologically 
      normal urothelium of patients with bladder cancer, although less frequently than 
      in hyperplasias and dysplasias. These findings support the hypothesis of 
      multi-focal development of bladder cancer from histologically inconspicuous but 
      already genetically altered urothelium. FISH analysis of chromosome 9 regions 
      could provide a useful tool to detect potentially premalignant lesions in the 
      follow-up care of patients with bladder cancer.
FAU - Obermann, Ellen C
AU  - Obermann EC
AD  - Institute of Pathology, University College London, London WC1E 6JJ, UK. 
      e.obermann@web.de
FAU - Meyer, Stefanie
AU  - Meyer S
FAU - Hellge, Dorothea
AU  - Hellge D
FAU - Zaak, Dirk
AU  - Zaak D
FAU - Filbeck, Thomas
AU  - Filbeck T
FAU - Stoehr, Robert
AU  - Stoehr R
FAU - Hofstaedter, Ferdinand
AU  - Hofstaedter F
FAU - Hartmann, Arndt
AU  - Hartmann A
FAU - Knuechel, Ruth
AU  - Knuechel R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
SB  - IM
MH  - *Aneuploidy
MH  - Biopsy
MH  - Chromosome Aberrations
MH  - *Chromosome Deletion
MH  - Chromosomes, Human, Pair 17/genetics
MH  - Chromosomes, Human, Pair 9/*genetics
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Urinary Bladder Neoplasms/diagnosis/*genetics/pathology
MH  - Urothelium/pathology
EDAT- 2004/03/11 05:00
MHDA- 2004/12/16 09:00
CRDT- 2004/03/11 05:00
PHST- 2004/03/11 05:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/03/11 05:00 [entrez]
PST - ppublish
SO  - Oncol Rep. 2004 Apr;11(4):745-51.