PMID- 15012739
OWN - NLM
STAT- MEDLINE
DCOM- 20050103
LR  - 20191108
IS  - 1320-5358 (Print)
IS  - 1320-5358 (Linking)
VI  - 8
IP  - 2
DP  - 2003 Apr
TI  - Glomerular expression of connective tissue growth factor mRNA in various renal 
      diseases.
PG  - 92-7
AB  - Connective tissue growth factor (CTGF) is a cysteine-rich member of a new family 
      of growth regulators. It is an important factor in the pathogenesis of mesangial 
      matrix accumulation and progressive glomerulosclerosis. The present study was 
      designed to elucidate the role of CTGF in diabetic nephropathy (DN), 
      immunoglobulin A nephropathy (IgA-N), membranous nephropathy (MN), and minimal 
      change nephrotic syndrome (MCNS). We evaluated the expression and localization of 
      CTGF mRNA in surgically excised renal tissue samples from 10 patients with DN, 10 
      with IgA-N, 10 with MN, 10 with MCNS, and 10 normal human kidney (NHK) tissue 
      samples, by using high-resolution in situ hybridization with digoxigenin-labelled 
      oligonucleotide. To quantify CTGF mRNA expression, we counted all nuclei, and 
      nuclei surrounded by CTGF-positive cytoplasm, in at least 10 randomly selected 
      cross-sections of non-sclerotic glomeruli, and expressed the results as a 
      percentage of total glomerular cells. In all glomeruli, CTGF mRNA was expressed 
      mainly in glomerular intrinsic cells, including glomerular mesangial and 
      epithelial cells and some cells of Bowman's capsule. The percentage of cells 
      positive for CTGF mRNA was significantly higher in DN and IgA-N than in MN, MCNS 
      and NHK. However, there was no significant difference in the percentage of CTGF 
      mRNA-positive cells between DN and IgA-N. Our study indicates that CTGF may play 
      an important role in the development and progression of glomerulosclerosis in DN 
      and IgA-N, which are both accompanied by mesangial matrix expansion and comprise 
      two major causes of end-stage renal failure.
FAU - Suzuki, Daisuke
AU  - Suzuki D
AD  - Division of Nephrology and Metabolism, Department of Internal Medicine, School of 
      Medicine, Tokai University, Isehara, Kanagawa, Japan. 
      daisuke@is.icc.u-tokyo.ac.jp
FAU - Toyoda, Masao
AU  - Toyoda M
FAU - Umezono, Tomoya
AU  - Umezono T
FAU - Uehara, Goro
AU  - Uehara G
FAU - Zhang, Shao-Yu
AU  - Zhang SY
FAU - Sakai, Takako
AU  - Sakai T
FAU - Nishina, Makoto
AU  - Nishina M
FAU - Suga, Takao
AU  - Suga T
FAU - Endoh, Masayuki
AU  - Endoh M
FAU - Yagame, Mitsunori
AU  - Yagame M
FAU - Sakai, Hideto
AU  - Sakai H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Nephrology (Carlton)
JT  - Nephrology (Carlton, Vic.)
JID - 9615568
RN  - 0 (CCN2 protein, human)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (RNA, Messenger)
RN  - 139568-91-5 (Connective Tissue Growth Factor)
SB  - IM
MH  - Adult
MH  - Connective Tissue Growth Factor
MH  - Female
MH  - Humans
MH  - Immediate-Early Proteins/*genetics
MH  - Intercellular Signaling Peptides and Proteins/*genetics
MH  - Kidney Diseases/*genetics
MH  - Kidney Glomerulus/*metabolism
MH  - Male
MH  - RNA, Messenger/*biosynthesis
EDAT- 2004/03/12 05:00
MHDA- 2005/01/04 09:00
CRDT- 2004/03/12 05:00
PHST- 2004/03/12 05:00 [pubmed]
PHST- 2005/01/04 09:00 [medline]
PHST- 2004/03/12 05:00 [entrez]
AID - 142 [pii]
AID - 10.1046/j.1440-1797.2003.00142.x [doi]
PST - ppublish
SO  - Nephrology (Carlton). 2003 Apr;8(2):92-7. doi: 10.1046/j.1440-1797.2003.00142.x.