PMID- 15013576
OWN - NLM
STAT- MEDLINE
DCOM- 20040511
LR  - 20061115
IS  - 0197-4580 (Print)
IS  - 0197-4580 (Linking)
VI  - 25
IP  - 4
DP  - 2004 Apr
TI  - Age-related changes in nitric oxide synthase and arginase in the rat prefrontal 
      cortex.
PG  - 547-52
AB  - Increasing evidence suggests that nitric oxide (NO), generated by nitric oxide 
      synthase (NOS) from l-arginine, plays an important role in the ageing process. 
      The present study, for the first time, investigates age-related changes in NOS 
      and arginase, an enzyme that shares a common substrate with NOS, in the 
      prefrontal cortex of rats assessed with and without prior behavioural testing. A 
      significant increase in total NOS activity was found in the prefrontal cortex in 
      aged (24-month-old) as compared with young (4-month-old) rats. Western blotting 
      revealed that there were no significant differences between young and aged rats 
      in neuronal NOS (nNOS) and endothelial NOS (eNOS) protein expression. Inducible 
      isoform of NOS (iNOS), in terms of activity and protein expression, was not 
      detected in either group. Total arginase activity and arginase I and II protein 
      expression did not differ between the young and aged groups. The present findings 
      support the contribution of NOS/NO to ageing but question the importance of iNOS 
      in the normal ageing process.
FAU - Liu, P
AU  - Liu P
AD  - Department of Pharmacology and Toxicology, School of Medical Sciences, Dunedin, 
      New Zealand. ping.liu@stonebow.otago.ac.nz
FAU - Smith, P F
AU  - Smith PF
FAU - Appleton, I
AU  - Appleton I
FAU - Darlington, C L
AU  - Darlington CL
FAU - Bilkey, D K
AU  - Bilkey DK
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neurobiol Aging
JT  - Neurobiology of aging
JID - 8100437
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 3.5.3.1 (Arginase)
SB  - IM
MH  - Aging/*metabolism
MH  - Animals
MH  - Arginase/*metabolism
MH  - Male
MH  - Nitric Oxide Synthase/*metabolism
MH  - Prefrontal Cortex/*enzymology
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2004/03/12 05:00
MHDA- 2004/05/12 05:00
CRDT- 2004/03/12 05:00
PHST- 2003/03/04 00:00 [received]
PHST- 2003/04/23 00:00 [revised]
PHST- 2003/07/23 00:00 [accepted]
PHST- 2004/03/12 05:00 [pubmed]
PHST- 2004/05/12 05:00 [medline]
PHST- 2004/03/12 05:00 [entrez]
AID - S0197458003001829 [pii]
AID - 10.1016/j.neurobiolaging.2003.07.003 [doi]
PST - ppublish
SO  - Neurobiol Aging. 2004 Apr;25(4):547-52. doi: 
      10.1016/j.neurobiolaging.2003.07.003.