PMID- 15015781 OWN - NLM STAT- MEDLINE DCOM- 20040401 LR - 20190911 IS - 0167-594X (Print) IS - 0167-594X (Linking) VI - 66 IP - 1-2 DP - 2004 Jan TI - Expression of somatostatin receptor mRNA in human meningiomas and their implication in in vitro antiproliferative activity. PG - 155-66 AB - Somatostatin receptors (SSTRs) have been detected in many normal and malignant tissues. This wide expression has been used for diagnostic, prognostic and therapeutic purposes. Five SSTR subtypes (SSTR 1-5) have been identified whose activation is responsible for the signal transduction through many different intracellular pathways. In the present study the expression of SSTR mRNA was determined by reverse-transcriptase (RT)-PCR in 42 meningiomas. About 88% of the tumors analyzed (37/42) were positive for at least one of the five SSTR subtypes displaying a variable pattern of expression of the different SSTR subtypes. SSTRI and SSTR2 were the most frequently mRNA detected (69% and 79% of the sample analyzed, respectively). The other subtypes were found in the 43%, 33% and 33% of cases for SSTR3, SSTR4 and SSTR5, respectively. In 22, out of 42 patients (52%) three or more SSTRs were detected. The expression of the different SSTR subtypes did not correlate with the expression of bcl-2 (apoptosis-associated protein) and MIB-1 (a proliferation marker), assessed by immunohistochemistry in a series of 34 tumor samples, while a correlation between the expression of SSTR3 and p53 was observed (p = 0.08). To evaluate a possible role of SSTR in the control of human meningioma cell proliferation, seven primary cell cultures obtained from fresh meningioma surgical tissues, were analyzed for their proliferative behavior by MTT assay and for their response to SST by [3H]-thymidine incorporation. In four out of six tumors (in one case no SSTR were detected) the treatment with SST caused a significant inhibition of DNA synthesis induced by the tumor-promoter phorbol myristate acetate. The evidence of the expression of SSTRs, mainly of SSTR2, in this series of specimens we analyzed altogether with in vitro antiproliferative effects of SST may open interesting perspectives for the diagnosis and the therapy of meningiomas. FAU - Arena, Sara AU - Arena S AD - Section of Pharmacology, Department of Oncology, Biology and Genetics, Genetics, University of Genova, Italy. FAU - Barbieri, Federica AU - Barbieri F FAU - Thellung, Stefano AU - Thellung S FAU - Pirani, Paolo AU - Pirani P FAU - Corsaro, Alessandro AU - Corsaro A FAU - Villa, Valentina AU - Villa V FAU - Dadati, Patrizia AU - Dadati P FAU - Dorcaratto, Alessandra AU - Dorcaratto A FAU - Lapertosa, Gabriella AU - Lapertosa G FAU - Ravetti, Jean-Louis AU - Ravetti JL FAU - Spaziante, Renato AU - Spaziante R FAU - Schettini, Gennaro AU - Schettini G FAU - Florio, Tullio AU - Florio T LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - J Neurooncol JT - Journal of neuro-oncology JID - 8309335 RN - 0 (Ki-67 Antigen) RN - 0 (Protein Isoforms) RN - 0 (Proto-Oncogene Proteins c-bcl-2) RN - 0 (RNA, Messenger) RN - 0 (Receptors, Somatostatin) RN - 0 (Tumor Suppressor Protein p53) SB - IM MH - Adult MH - Aged MH - Aged, 80 and over MH - Cell Division MH - Female MH - Gene Expression MH - Humans MH - Immunohistochemistry MH - Ki-67 Antigen/metabolism MH - Male MH - Meningeal Neoplasms/*metabolism/*pathology MH - Meningioma/*metabolism/*pathology MH - Middle Aged MH - Protein Isoforms/metabolism MH - Proto-Oncogene Proteins c-bcl-2/metabolism MH - RNA, Messenger/metabolism MH - Receptors, Somatostatin/genetics/*metabolism MH - Reverse Transcriptase Polymerase Chain Reaction MH - Tumor Cells, Cultured MH - Tumor Suppressor Protein p53/metabolism EDAT- 2004/03/16 05:00 MHDA- 2004/04/02 05:00 CRDT- 2004/03/16 05:00 PHST- 2004/03/16 05:00 [pubmed] PHST- 2004/04/02 05:00 [medline] PHST- 2004/03/16 05:00 [entrez] AID - 10.1023/b:neon.0000013498.19981.55 [doi] PST - ppublish SO - J Neurooncol. 2004 Jan;66(1-2):155-66. doi: 10.1023/b:neon.0000013498.19981.55.