PMID- 15016162
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20040630
LR  - 20040312
IS  - 1399-543X (Print)
IS  - 1399-543X (Linking)
VI  - 3
IP  - 2
DP  - 2002 Jun
TI  - DNA extraction and HLA genotyping using mailed mouth brushes from children.
PG  - 89-94
AB  - The need for blood samples in genetic epidemiologic studies often leads to low 
      response rate among non-diseased individuals, and the collection of blood samples 
      is costly and labor-intensive. We tested the feasibility of extracting DNA for 
      human leukocyte antigen (HLA) typing from buccal cells collected with mailed, 
      self-administered mouth brushes. A random sample of 1474 Norwegian children aged 
      0-17 yr was contacted by mail and received information about the study and mouth 
      brushes for buccal cell samples. Brushes were returned by mail, DNA was extracted 
      and the HLA-DQA1 and -DQB1 allelic polymorphisms were determined using polymerase 
      chain reaction (PCR) and sequence-specific oligonucleotide probes. Mouth swabs 
      were returned from 1068 (72.5%). Of these, DNA was extracted and HLA typing 
      successfully completed for 1056 individuals (98.9%). In conclusion, we have 
      described an efficient and safe set of methods for application in genetic 
      epidemiologic studies of type 1 diabetes and other HLA-related diseases. A large 
      proportion of randomly selected children returned self-administered mouth swabs 
      with DNA of sufficient quality and quantity for HLA genotyping.
FAU - Witso, Elisabet
AU  - Witso E
AD  - Division of Epidemiology, Norwegian Institute of Public Health, Oslo, Norway.
FAU - Stene, Lars C
AU  - Stene LC
FAU - Paltiel, Liv
AU  - Paltiel L
FAU - Joner, Geir
AU  - Joner G
FAU - Ronningen, Kjersti S
AU  - Ronningen KS
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Pediatr Diabetes
JT  - Pediatric diabetes
JID - 100939345
EDAT- 2004/03/16 05:00
MHDA- 2004/03/16 05:01
CRDT- 2004/03/16 05:00
PHST- 2004/03/16 05:00 [pubmed]
PHST- 2004/03/16 05:01 [medline]
PHST- 2004/03/16 05:00 [entrez]
AID - PDIpdi030205 [pii]
AID - 10.1034/j.1399-5448.2002.30205.x [doi]
PST - ppublish
SO  - Pediatr Diabetes. 2002 Jun;3(2):89-94. doi: 10.1034/j.1399-5448.2002.30205.x.