PMID- 15016199
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20040630
LR  - 20220321
IS  - 1399-543X (Print)
IS  - 1399-543X (Linking)
VI  - 2
IP  - 2
DP  - 2001 Jun
TI  - Prediction of silent celiac disease at diagnosis of childhood type 1 diabetes by 
      tissue transglutaminase autoantibodies and HLA.
PG  - 58-65
AB  - AIMS: The aims were to estimate the diagnostic sensitivity and specificity of 
      autoantibodies to tissue transglutaminase (IgA- and IgG-tTG), gliadin (AGA) and 
      endomysium (EMA) in relation to human leukocyte antigen (HLA)-DQB1 alleles to 
      identify silent celiac disease at diagnosis of type 1 diabetes. METHODS: IgA- and 
      IgG-tTG were measured in radioligand binding assays in 165 type 1 diabetic 
      patients. Data on HLA-DQB1 were available for 148 patients and on both AGA and 
      EMA for 164 patients. For patients considered positive for AGA or EMA, or both, 
      an intestinal biopsy was suggested. HLA-DQB1 typing was carried out by polymerase 
      chain reaction and hybridization with allele specific probes. RESULTS: Three 
      patients, left out from further study of antibodies, but not from HLA-DQB1 
      analysis, had treated celiac disease at diagnosis. Out of the other 162 type 1 
      diabetic patients tested, nine had IgA-tTG, six IgG-tTG, eight EMA, and 11 AGA. 
      Biopsy was suggested for nine patients, of whom six showed villous atrophy, one 
      did not and two refused to participate. Thus, silent celiac disease was probable 
      in 8/162 and biopsy-verified in 6/162, where five patients were AGA-positive and 
      six either EMA-, IgA-tTG- or IgG-tTG-positive. Of the 11 patients with celiac 
      disease (three with treated and eight with silent celiac disease), 10 were 
      HLA-DQB1-typed, of whom 65% (13/20) had the DQB1*02 allele, compared with 36% 
      (100/276; p = 0.011) of those without celiac disease. IgA-tTG levels were higher 
      in patients having either *02 or *0302 (0.6; -1.3-112.4 RU) compared with those 
      not having these alleles (0.4; -0.7-3.4 RU; p = 0.023). CONCLUSION: IgA-tTG are 
      HLA-DQB1*02-associated autoantibodies with high sensitivity and specificity for 
      silent celiac disease at diagnosis of type 1 diabetes.
FAU - Agardh, D
AU  - Agardh D
AD  - Department of Pediatrics, Malmo University Hospital, Lund University, Malmo, 
      Sweden. daniel.agardh@home.se
FAU - Nilsson, A
AU  - Nilsson A
FAU - Tuomi, T
AU  - Tuomi T
FAU - Lindberg, B
AU  - Lindberg B
FAU - Carlsson, A K
AU  - Carlsson AK
FAU - Lernmark, A
AU  - Lernmark A
FAU - Ivarsson, S A
AU  - Ivarsson SA
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Pediatr Diabetes
JT  - Pediatric diabetes
JID - 100939345
EDAT- 2004/03/16 05:00
MHDA- 2004/03/16 05:01
CRDT- 2004/03/16 05:00
PHST- 2004/03/16 05:00 [pubmed]
PHST- 2004/03/16 05:01 [medline]
PHST- 2004/03/16 05:00 [entrez]
AID - PDI020203 [pii]
AID - 10.1034/j.1399-5448.2001.002002058.x [doi]
PST - ppublish
SO  - Pediatr Diabetes. 2001 Jun;2(2):58-65. doi: 10.1034/j.1399-5448.2001.002002058.x.