PMID- 15016361
OWN - NLM
STAT- MEDLINE
DCOM- 20041027
LR  - 20131121
IS  - 0969-2126 (Print)
IS  - 0969-2126 (Linking)
VI  - 12
IP  - 3
DP  - 2004 Mar
TI  - Noncatalytic assembly of ribonuclease III with double-stranded RNA.
PG  - 457-66
AB  - Ribonuclease III (RNase III) represents a family of double-stranded RNA (dsRNA) 
      endonucleases. The simplest bacterial enzyme contains an endonuclease domain 
      (endoND) and a dsRNA binding domain (dsRBD). RNase III can affect RNA structure 
      and gene expression in either of two ways: as a dsRNA-processing enzyme that 
      cleaves dsRNA, or as a dsRNA binding protein that binds but does not cleave 
      dsRNA. We previously determined the endoND structure of Aquifex aeolicus RNase 
      III (Aa-RNase III) and modeled a catalytic complex of full-length Aa-RNase III 
      with dsRNA. Here, we present the crystal structure of Aa-RNase III in complex 
      with dsRNA, revealing a noncatalytic assembly. The major differences between the 
      two functional forms of RNase III.dsRNA are the conformation of the protein and 
      the orientation and location of dsRNA. The flexibility of a 7 residue linker 
      between the endoND and dsRBD enables the transition between these two forms.
FAU - Blaszczyk, Jaroslaw
AU  - Blaszczyk J
AD  - Center for Cancer Research, National Cancer Institute, National Institutes of 
      Health, Frederick, MD 21702 USA.
FAU - Gan, Jianhua
AU  - Gan J
FAU - Tropea, Joseph E
AU  - Tropea JE
FAU - Court, Donald L
AU  - Court DL
FAU - Waugh, David S
AU  - Waugh DS
FAU - Ji, Xinhua
AU  - Ji X
LA  - eng
SI  - PDB/1RC5
SI  - PDB/1RC7
PT  - Journal Article
PL  - United States
TA  - Structure
JT  - Structure (London, England : 1993)
JID - 101087697
RN  - 0 (RNA, Double-Stranded)
RN  - EC 3.1.26.3 (Ribonuclease III)
RN  - I38ZP9992A (Magnesium)
SB  - IM
CIN - Structure. 2004 Apr;12(4):520-1. PMID: 15062073
MH  - Amino Acid Sequence
MH  - Binding Sites
MH  - Magnesium/metabolism
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Protein Structure, Quaternary
MH  - Protein Structure, Tertiary
MH  - RNA, Double-Stranded/*metabolism
MH  - Ribonuclease III/chemistry/genetics/*metabolism
EDAT- 2004/03/16 05:00
MHDA- 2004/10/28 09:00
CRDT- 2004/03/16 05:00
PHST- 2003/10/10 00:00 [received]
PHST- 2003/11/04 00:00 [revised]
PHST- 2003/11/14 00:00 [accepted]
PHST- 2004/03/16 05:00 [pubmed]
PHST- 2004/10/28 09:00 [medline]
PHST- 2004/03/16 05:00 [entrez]
AID - S0969212604000462 [pii]
AID - 10.1016/j.str.2004.02.004 [doi]
PST - ppublish
SO  - Structure. 2004 Mar;12(3):457-66. doi: 10.1016/j.str.2004.02.004.