PMID- 15016889
OWN - NLM
STAT- MEDLINE
DCOM- 20040408
LR  - 20240314
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 78
IP  - 7
DP  - 2004 Apr
TI  - Characterization of emerging European-like porcine reproductive and respiratory 
      syndrome virus isolates in the United States.
PG  - 3684-703
AB  - European-like field isolates of porcine reproductive and respiratory syndrome 
      virus (PRRSV) have recently emerged in North America. The full-length genomic 
      sequence of an index isolate characterized in 1999, strain EuroPRRSV, served as 
      the reference strain for further studies of the evolution and epidemiology of 
      European-like isolates (type 1) in the United States. Strain EuroPRRSV shared 
      90.1 to 100% amino acid identity with the prototype European strain, Lelystad, 
      within the structural and nonstructural open reading frames (ORFs) and 95.3% 
      overall nucleotide identity. The 5' untranslated region and two nonstructural 
      regions within ORF 1 were closely examined due to significant divergence from 
      strain Lelystad. A 51-bp deletion in a region within ORF 1a, coding for 
      nonstructural protein 2 (NSP2), was observed. Sequence analysis of the structural 
      ORFs 2 to 7 of additional European-like isolates indicated that these isolates 
      share 93% nucleotide identity with one another and 95 to 96% identity with the 
      Lelystad strain but only 70% identity with the North American reference strain 
      VR-2332. Phylogenetic analysis with published PRRSV ORF 3, 5, and 7 nucleotide 
      sequences indicated that these newly emerging isolates form a clade with the 
      Lelystad and United Kingdom PRRSV isolates. Detailed analysis of four of these 
      isolates with a panel of 60 monoclonal antibodies directed against the structural 
      proteins confirmed a recognition pattern that was more consistent with strain 
      Lelystad than with other North American isolates.
FAU - Ropp, Susan L
AU  - Ropp SL
AD  - Department of Veterinary Science, South Dakota State University, Brookings, South 
      Dakota, USA.
FAU - Wees, Carrie E Mahlum
AU  - Wees CE
FAU - Fang, Ying
AU  - Fang Y
FAU - Nelson, Eric A
AU  - Nelson EA
FAU - Rossow, Kurt D
AU  - Rossow KD
FAU - Bien, Melissa
AU  - Bien M
FAU - Arndt, Bill
AU  - Arndt B
FAU - Preszler, Sarah
AU  - Preszler S
FAU - Steen, Pamela
AU  - Steen P
FAU - Christopher-Hennings, Jane
AU  - Christopher-Hennings J
FAU - Collins, James E
AU  - Collins JE
FAU - Benfield, David A
AU  - Benfield DA
FAU - Faaberg, Kay S
AU  - Faaberg KS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antigens, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, Viral/chemistry/genetics
MH  - Base Sequence
MH  - Europe
MH  - Genes, Viral/genetics
MH  - Genetic Variation/genetics
MH  - *Genome, Viral
MH  - Molecular Sequence Data
MH  - Open Reading Frames/genetics
MH  - Porcine Reproductive and Respiratory Syndrome/*epidemiology/*virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/chemistry/*genetics/*isolation & purification
MH  - Sequence Analysis, DNA
MH  - Swine/*virology
MH  - United States/epidemiology
MH  - Viral Proteins/chemistry/genetics
PMC - PMC371078
EDAT- 2004/03/16 05:00
MHDA- 2004/04/09 05:00
PMCR- 2004/04/01
CRDT- 2004/03/16 05:00
PHST- 2004/03/16 05:00 [pubmed]
PHST- 2004/04/09 05:00 [medline]
PHST- 2004/03/16 05:00 [entrez]
PHST- 2004/04/01 00:00 [pmc-release]
AID - 2084 [pii]
AID - 10.1128/jvi.78.7.3684-3703.2004 [doi]
PST - ppublish
SO  - J Virol. 2004 Apr;78(7):3684-703. doi: 10.1128/jvi.78.7.3684-3703.2004.