PMID- 15017977
OWN - NLM
STAT- MEDLINE
DCOM- 20040324
LR  - 20190701
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 74
IP  - 18
DP  - 2004 Mar 19
TI  - Regulation of the cholinergic gene locus by the repressor element-1 silencing 
      transcription factor/neuron restrictive silencer factor (REST/NRSF).
PG  - 2213-25
AB  - The cholinergic gene locus is comprised of two genes, the choline 
      acetyltransferase gene and the vesicular acetylcholine transporter gene. The 
      vesicular acetylcholine transporter gene is located within the first intron of 
      the choline acetyltransferase gene. This arrangement permits coordinate 
      regulation of the locus. Protein kinase A regulates expression of the cholinergic 
      gene locus in PC12 cells. This regulation was found to be dependent on the 
      presence of a 21-bp DNA sequence known as the repressor element- (RE- 
      1)/neuron-restrictive silencer element(NRSE). Repressor element-I silencing 
      transcription factor (REST)/ neuron-restrictive silencer factor (NRSF), which 
      binds to the RE-I/NRSE, is a zinc finger containing transcriptional repressor 
      that blocks the expression of many neuronal RE-I/NRSE containing genes in 
      nonneuronal cells. However, REST/NRSF expression has also been observed in 
      neurons as well as the PC 12 cell line used in these studies. REST/NRSF truncated 
      isoforms were expressed in neuronal cells, suggesting they also function in 
      regulating neuronal gene expression. A study of REST4, one of the REST/NRSF 
      isoforms, suggests that it regulates transcription of the cholinergic gene locus 
      by blocking the repressor activity of REST/NRSF. Protein kinase A regulation of 
      the cholinergic gene locus in PC 12 cells can thus be attributed, at least in 
      part, to increased synthesis of REST4, which in turn derepresses the repressor 
      activity of REST/NRSF.
FAU - Shimojo, Masahito
AU  - Shimojo M
AD  - Department of Molecular and Cellular Biochemistry, University of Kentucky, 
      Chandler Medical Center, Lexington, KY 40536-0298, USA.
FAU - Hersh, Louis B
AU  - Hersh LB
LA  - eng
GR  - AG05893/AG/NIA NIH HHS/United States
GR  - AG46734/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Review
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Carrier Proteins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (RE1-silencing transcription factor)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (Vesicular Acetylcholine Transport Proteins)
RN  - 0 (Vesicular Transport Proteins)
RN  - EC 2.3.1.6 (Choline O-Acetyltransferase)
SB  - IM
MH  - Animals
MH  - Carrier Proteins/*genetics
MH  - Choline O-Acetyltransferase/*genetics
MH  - Gene Components
MH  - *Gene Expression Regulation
MH  - *Membrane Transport Proteins
MH  - Neurons/*metabolism
MH  - Protein Isoforms/physiology
MH  - Protein Structure, Tertiary
MH  - Repressor Proteins/chemistry/*physiology
MH  - Transcription Factors/chemistry/*physiology
MH  - Transcription, Genetic
MH  - Vesicular Acetylcholine Transport Proteins
MH  - *Vesicular Transport Proteins
MH  - Zinc Fingers
RF  - 63
EDAT- 2004/03/17 05:00
MHDA- 2004/03/25 05:00
CRDT- 2004/03/17 05:00
PHST- 2004/03/17 05:00 [pubmed]
PHST- 2004/03/25 05:00 [medline]
PHST- 2004/03/17 05:00 [entrez]
AID - S0024-3205(03)01169-X [pii]
AID - 10.1016/j.lfs.2003.08.045 [doi]
PST - ppublish
SO  - Life Sci. 2004 Mar 19;74(18):2213-25. doi: 10.1016/j.lfs.2003.08.045.