PMID- 15018635
OWN - NLM
STAT- MEDLINE
DCOM- 20040616
LR  - 20220409
IS  - 1471-2334 (Electronic)
IS  - 1471-2334 (Linking)
VI  - 4
DP  - 2004 Feb 13
TI  - Association of Atopobium vaginae, a recently described metronidazole resistant 
      anaerobe, with bacterial vaginosis.
PG  - 5
AB  - BACKGROUND: Bacterial vaginosis (BV) is a polymicrobial syndrome characterized by 
      a change in vaginal flora away from predominantly Lactobacillus species. The 
      cause of BV is unknown, but the condition has been implicated in diverse medical 
      outcomes. The bacterium Atopobium vaginae has been recognized only recently. It 
      is not readily identified by commercial diagnostic kits. Its clinical 
      significance is unknown but it has recently been isolated from a tuboovarian 
      abcess. METHODS: Nucleotide sequencing of PCR amplified 16S rRNA gene segments, 
      that were separated into bands within lanes on polyacrylamide gels by denaturing 
      gradient gel electrophoresis (DGGE), was used to examine bacterial vaginal flora 
      in 46 patients clinically described as having normal (Lactobacillus spp. 
      predominant; Nugent score < or = 3) and abnormal flora (Nugent score > or = 4). 
      These women ranged in age from 14 to 48 and 82% were African American. RESULTS: 
      The DGGE banding patterns of normal and BV-positive patients were recognizably 
      distinct. Those of normal patients contained 1 to 4 bands that were focused in 
      the centre region of the gel lane, while those of BV positive patients contained 
      bands that were not all focused in the center region of the gel lane. More 
      detailed analysis of patterns revealed that bands identified as Atopobium vaginae 
      were present in a majority (12/22) of BV positive patients, while corresponding 
      bands were rare (2/24) in normal patients. (P < 0.001) Two A. vaginae isolates 
      were cultivated from two patients whose DGGE analyses indicated the presence of 
      this organism. Two A. vaginae 16S rRNA gene sequences were identified among the 
      clinical isolates. The same two sequences were obtained from DGGE bands of the 
      corresponding vaginal flora. The sequences differed by one nucleotide over the 
      short (approximately 300 bp) segment used for DGGE analysis and migrated to 
      slightly different points in denaturing gradient gels. Both isolates were strict 
      anaerobes and highly metronidazole resistant. CONCLUSION: The results suggest 
      that A. vaginae may be an important component of the complex bacterial ecology 
      that constitutes abnormal vaginal flora. This organism could play a role in 
      treatment failure if further studies confirm it is consistently metronidozole 
      resistant.
FAU - Ferris, Michael J
AU  - Ferris MJ
AD  - Dept of Pediatrics, Louisiana State University Health Sciences Center, New 
      Orleans, LA 70112, USA. mferris@chnola-research.org
FAU - Masztal, Alicia
AU  - Masztal A
FAU - Aldridge, Kenneth E
AU  - Aldridge KE
FAU - Fortenberry, J Dennis
AU  - Fortenberry JD
FAU - Fidel, Paul L Jr
AU  - Fidel PL Jr
FAU - Martin, David H
AU  - Martin DH
LA  - eng
GR  - U19 AI43024/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20040213
PL  - England
TA  - BMC Infect Dis
JT  - BMC infectious diseases
JID - 100968551
RN  - 0 (Anti-Infective Agents)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 140QMO216E (Metronidazole)
SB  - IM
MH  - Actinobacteria/*drug effects/genetics/isolation & purification
MH  - Adolescent
MH  - Adult
MH  - Anti-Infective Agents/*pharmacology
MH  - Bacteria, Anaerobic
MH  - Drug Resistance, Bacterial
MH  - Female
MH  - Humans
MH  - Metronidazole/*pharmacology
MH  - Microbial Sensitivity Tests
MH  - Middle Aged
MH  - Pregnancy
MH  - RNA, Ribosomal, 16S/analysis
MH  - Vaginosis, Bacterial/*microbiology
PMC - PMC362875
EDAT- 2004/03/17 05:00
MHDA- 2004/06/17 05:00
PMCR- 2004/02/13
CRDT- 2004/03/17 05:00
PHST- 2003/10/28 00:00 [received]
PHST- 2004/02/13 00:00 [accepted]
PHST- 2004/03/17 05:00 [pubmed]
PHST- 2004/06/17 05:00 [medline]
PHST- 2004/03/17 05:00 [entrez]
PHST- 2004/02/13 00:00 [pmc-release]
AID - 1471-2334-4-5 [pii]
AID - 10.1186/1471-2334-4-5 [doi]
PST - epublish
SO  - BMC Infect Dis. 2004 Feb 13;4:5. doi: 10.1186/1471-2334-4-5.