PMID- 15018660
OWN - NLM
STAT- MEDLINE
DCOM- 20040511
LR  - 20220408
IS  - 0882-8245 (Print)
IS  - 0882-8245 (Linking)
VI  - 17
IP  - 1
DP  - 2004
TI  - Impact of immunizations with porcine reproductive and respiratory syndrome virus 
      on lymphoproliferative recall responses of CD8+ T cells.
PG  - 25-37
AB  - The objective of this study was to investigate the immune responses elicited by 
      either a modified-live (MLV) or a killed virus (KV) porcine reproductive and 
      respiratory syndrome virus (PRRSV) vaccine. Specifically, we investigated the 
      effects of multiple vaccinations on antigen-specific cellular and antibody 
      responses against PRRSV. Twelve sows were obtained from herds with either a 
      history of repeated MLV or KV PRRSV vaccination and a non-vaccinated, 
      PRRSV-negative herd. Within herd, sows were divided into three groups and 
      vaccinated with MLV, KV, or injected with saline. On day 0, 27, and 38, recall 
      responses of peripheral blood mononuclear cells (PBMC) to the parent strains of 
      the vaccines (e.g., MLV-VR2332 or KV-ISUP) were examined. The concentrations of 
      total PRRSV-specific and virus-neutralizing serum antibodies were determined by 
      ELISA and serum neutralization assays. Following immunization, the 
      antigen-specific proliferation of CD8alphabeta(+), CD4(+)CD8alphaalpha(+) T cells 
      in the naive sows was greater than in sows repeatedly vaccinated with KV or MLV. 
      This diminished lymphoproliferative responses of CD8alphabeta(+) and 
      CD4(+)CD8alphaalpha(+) T cells could be partially overcome by heterologous 
      immunization. However, B cell proliferation, PRRSV antibody concentrations and 
      virus neutralizing antibody titers were not enhanced by heterologous immunization 
      and only KV vaccination increased antibody levels in previously immunized (MLV or 
      KV) sows.
FAU - Bassaganya-Riera, Josep
AU  - Bassaganya-Riera J
AD  - Department of Veterinary Microbiology and Preventive Medicine, Iowa State 
      University, Ames, Iowa, USA. jbassaga@vt.edu
FAU - Thacker, Brad J
AU  - Thacker BJ
FAU - Yu, Shan
AU  - Yu S
FAU - Strait, Erin
AU  - Strait E
FAU - Wannemuehler, Michael J
AU  - Wannemuehler MJ
FAU - Thacker, Eileen L
AU  - Thacker EL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Viral Immunol
JT  - Viral immunology
JID - 8801552
RN  - 0 (Antibodies, Viral)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Female
MH  - Lymphocyte Activation/*immunology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Swine
MH  - Vaccination
MH  - Vaccines, Attenuated/administration & dosage/immunology
MH  - Viral Vaccines/*administration & dosage/immunology
EDAT- 2004/03/17 05:00
MHDA- 2004/05/12 05:00
CRDT- 2004/03/17 05:00
PHST- 2004/03/17 05:00 [pubmed]
PHST- 2004/05/12 05:00 [medline]
PHST- 2004/03/17 05:00 [entrez]
AID - 10.1089/088282404322875430 [doi]
PST - ppublish
SO  - Viral Immunol. 2004;17(1):25-37. doi: 10.1089/088282404322875430.