PMID- 15019725
OWN - NLM
STAT- MEDLINE
DCOM- 20041029
LR  - 20040315
IS  - 0890-6238 (Print)
IS  - 0890-6238 (Linking)
VI  - 18
IP  - 2
DP  - 2004 Mar-Apr
TI  - Telemetric uterine contraction model in preterm cynomolgus monkeys.
PG  - 285-93
AB  - A telemetric-based model is presented for evaluation of uterine contractions and 
      preterm labor (PTL) in pregnant cynomolgus monkeys. The model allows continuous 
      monitoring of electromyography (EMG) and intrauterine pressure (IUP) as 
      indicators of uterine activity. A pressure sensor was implanted into the amnion 
      of pregnant monkeys on gestational day (GD) 120 +/- 3 and biopotential sensors 
      were attached to the uterus. A telemetry transmitter was placed in a subcuticular 
      pocket located in the flank. Venous catheters were tethered to the next room for 
      dosing and blood sampling without disturbing the conscious animals. EMG and/or 
      IUP were monitored continuously post-operatively. IUP is a reliable parameter for 
      monitoring intrauterine activity, as demonstrated by a close relationship between 
      bursts of activity in the EMG and increases in IUP. Animals close to term showed 
      a basal level of uterine activity during the daytime, with irregular contractions 
      of <10 mmHg. In the night, spontaneous contractions (10-40 mmHg; maximum between 
      18:00 and 01:00 h) appeared every 3-6 min. Artificial contractions of 15-40 mmHg 
      that mimicked preterm labor were induced at any time of the day by infusion of 
      5-60 mU oxytocin (OT) per kilogram per hour. These contractions showed a 
      dose-dependent response to OT, and were stable for up to 14 h of constant 
      infusion of OT. Following withdrawal of oxytocin, contractions returned to 
      baseline within 1h. No desensitization of oxytocin-induced contractions was 
      observed when oxytocin was administered daily for up to several weeks. This 
      telemetric model characterizes uterine contractions in non-human primates and 
      provides an excellent method to evaluate pharmacological characteristics of drug 
      candidates intended to treat PTL.
FAU - Chellman, Gary J
AU  - Chellman GJ
AD  - Charles River Discovery and Development Services, Sierra Division, 587 Dunn 
      Circle, Sparks, NV 89431, USA. gchellman@sbi.criver.com
FAU - Bee, Walter H
AU  - Bee WH
FAU - Resendez, John C
AU  - Resendez JC
FAU - Meyer, Julie K
AU  - Meyer JK
FAU - Bernal, Jan
AU  - Bernal J
FAU - Moddrelle, David S
AU  - Moddrelle DS
FAU - Haluska, George J
AU  - Haluska GJ
FAU - Reinheimer, Torsten M
AU  - Reinheimer TM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Reprod Toxicol
JT  - Reproductive toxicology (Elmsford, N.Y.)
JID - 8803591
RN  - 50-56-6 (Oxytocin)
SB  - IM
MH  - Animals
MH  - Catheters, Indwelling
MH  - Electrodes, Implanted
MH  - Electromyography
MH  - Female
MH  - Macaca fascicularis
MH  - *Models, Animal
MH  - Oxytocin/pharmacology
MH  - Pregnancy
MH  - Pressure
MH  - *Telemetry/instrumentation
MH  - *Uterine Contraction
MH  - Uterine Monitoring
EDAT- 2004/03/17 05:00
MHDA- 2004/10/30 09:00
CRDT- 2004/03/17 05:00
PHST- 2003/09/09 00:00 [received]
PHST- 2003/12/02 00:00 [revised]
PHST- 2003/12/15 00:00 [accepted]
PHST- 2004/03/17 05:00 [pubmed]
PHST- 2004/10/30 09:00 [medline]
PHST- 2004/03/17 05:00 [entrez]
AID - S0890623803001680 [pii]
AID - 10.1016/j.reprotox.2003.12.002 [doi]
PST - ppublish
SO  - Reprod Toxicol. 2004 Mar-Apr;18(2):285-93. doi: 10.1016/j.reprotox.2003.12.002.