PMID- 15019840
OWN - NLM
STAT- MEDLINE
DCOM- 20040422
LR  - 20181109
IS  - 0003-9861 (Print)
IS  - 0003-9861 (Linking)
VI  - 424
IP  - 1
DP  - 2004 Apr 1
TI  - L-Glutamate in the extracellular space regulates endogenous D-aspartate 
      homeostasis in rat pheochromocytoma MPT1 cells.
PG  - 89-96
AB  - In previous studies [FEBS Lett. 434 (1998) 231, Arch. Biochem. Biophys. 404 
      (2002) 92], we demonstrated for the first time that D-aspartate (D-Asp) is 
      synthesized in cultured mammalian cell lines, such as pheochromocytoma 12 (PC12) 
      and its subclone, MPT1. Our current focus is analysis of the dynamics of D-Asp 
      homeostasis in these cells. In this communication, we show that L-glutamate (Glu) 
      and L-Glu transporter substrates in the extracellular space regulate the 
      homeostasis of endogenous D-Asp in MPT1 cells. D-Asp is apparently in dynamic 
      homeostasis, whereby endogenous D-Asp is constantly released into the 
      extracellular space by an undefined mechanism, and continuously and intensively 
      taken up into cells by an L-Glu transporter. Under these conditions, L-Glu and 
      its transporter substrates in the medium may competitively inhibit the uptake of 
      D-Asp via the transporter, resulting in accumulation of the amino acid in the 
      extracellular space. We additionally demonstrate that DL-TBOA, a well-established 
      L-Glu transporter inhibitor, is taken up by the transporter during long time 
      intervals, but not on a short time-scale.
FAU - Adachi, Minako
AU  - Adachi M
AD  - School of Pharmaceutical Sciences, Kitasato University, 5-9-1 Shirokane, 
      Minato-ku, Tokyo 108-8641, Japan.
FAU - Koyama, Hayato
AU  - Koyama H
FAU - Long, Zhiqun
AU  - Long Z
FAU - Sekine, Masae
AU  - Sekine M
FAU - Furuchi, Takemitsu
AU  - Furuchi T
FAU - Imai, Kazuhiro
AU  - Imai K
FAU - Nimura, Noriyuki
AU  - Nimura N
FAU - Shimamoto, Keiko
AU  - Shimamoto K
FAU - Nakajima, Terumi
AU  - Nakajima T
FAU - Homma, Hiroshi
AU  - Homma H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 0 (Amino Acid Transport System X-AG)
RN  - 0 (Neurotransmitter Agents)
RN  - 0 (benzyloxyaspartate)
RN  - 30KYC7MIAI (Aspartic Acid)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - K848JZ4886 (Cysteine)
RN  - UZY4HYK4ZX (cysteine sulfinic acid)
SB  - IM
MH  - Amino Acid Transport System X-AG/antagonists & inhibitors/metabolism
MH  - Animals
MH  - Aspartic Acid/analogs & derivatives/*metabolism/pharmacology
MH  - Cell Line, Tumor
MH  - Cysteine/*analogs & derivatives/metabolism/pharmacology
MH  - Extracellular Space/metabolism
MH  - Glutamic Acid/*metabolism/pharmacology
MH  - Homeostasis
MH  - Neurotransmitter Agents
MH  - PC12 Cells
MH  - Pheochromocytoma/metabolism
MH  - Rats
EDAT- 2004/03/17 05:00
MHDA- 2004/04/23 05:00
CRDT- 2004/03/17 05:00
PHST- 2003/12/26 00:00 [received]
PHST- 2004/01/24 00:00 [revised]
PHST- 2004/03/17 05:00 [pubmed]
PHST- 2004/04/23 05:00 [medline]
PHST- 2004/03/17 05:00 [entrez]
AID - S0003986104000529 [pii]
AID - 10.1016/j.abb.2004.01.016 [doi]
PST - ppublish
SO  - Arch Biochem Biophys. 2004 Apr 1;424(1):89-96. doi: 10.1016/j.abb.2004.01.016.