PMID- 15024009
OWN - NLM
STAT- MEDLINE
DCOM- 20040708
LR  - 20231213
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 279
IP  - 23
DP  - 2004 Jun 4
TI  - Isolation and characterization of the early nodule-specific protein homologue 
      (Hev b 13), an allergenic lipolytic esterase from Hevea brasiliensis latex.
PG  - 23933-41
AB  - Recurring reports of a highly allergenic 42-46-kDa protein in Hevea brasiliensis 
      latex appeared to have been resolved with the discovery of a 43-kDa allergenic 
      latex protein that was a homologue to patatin. However, the low to moderate 
      prevalence of sensitization to the protein, designated Hev b 7, among 
      latex-allergic patients could not adequately explain the frequent observations of 
      the 42-46-kDa allergen. This led to the hypothesis that another, more allergenic 
      protein of a similar molecular mass existed in Hevea latex. We report the 
      isolation and purification of a 42.98-kDa latex glycoprotein showing homology to 
      the early nodule-specific protein (ENSP) of the legumes Medicago sativa, Medicago 
      truncatula, and Glycine max. The protein is allergenic, being recognized by 
      immunoglobulin E (IgE) in sera from latex-allergic patients. The IgE epitope 
      resides on the carbohydrate moiety of the protein, and the presence of a similar 
      carbohydrate component on potato tuber patatin enables the latter to inhibit IgE 
      binding to the ENSP homologue. The cDNA encoding the ENSP homologue was isolated 
      by reverse transcription-PCR and cloned. The protein predicted from the cDNA 
      sequence has 391 amino acids, the first 26 of which constitute a putative signal 
      peptide. The deduced molecular mass of the mature protein is 40.40 kDa, while its 
      isoelectric point is estimated at 5.0. The discrepancy between the predicted and 
      observed molecular mass might be due to glycosylation, for which three N-sites on 
      the protein are predicted. The purified protein showed lipase and esterase 
      activities and may be involved in plant defense.
FAU - Arif, Siti Arija M
AU  - Arif SA
AD  - Rubber Research Institute of Malaysia, 50450 Kuala Lumpur, Malaysia. 
      sitiarija@lgm.gov.my
FAU - Hamilton, Robert G
AU  - Hamilton RG
FAU - Yusof, Faridah
AU  - Yusof F
FAU - Chew, Nyu-Ping
AU  - Chew NP
FAU - Loke, Yin-Ho
AU  - Loke YH
FAU - Nimkar, Subodh
AU  - Nimkar S
FAU - Beintema, Jaap J
AU  - Beintema JJ
FAU - Yeang, Hoong-Yeet
AU  - Yeang HY
LA  - eng
SI  - GENBANK/AY283800
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040315
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Allergens)
RN  - 0 (Antigens, Plant)
RN  - 0 (Carbohydrates)
RN  - 0 (DNA, Complementary)
RN  - 0 (Epitopes)
RN  - 0 (Hev b 13 allergen, Hevea brasiliensis)
RN  - 0 (Latex)
RN  - 0 (Peptides)
RN  - 0 (Plant Proteins)
RN  - 0 (Protein Sorting Signals)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9007-49-2 (DNA)
RN  - EC 3.1.- (Esterases)
RN  - EC 3.1.1.3 (Lipase)
RN  - EC 3.4.21.4 (Trypsin)
SB  - IM
MH  - Allergens/*chemistry/*isolation & purification
MH  - Amino Acid Sequence
MH  - Antigens, Plant
MH  - Base Sequence
MH  - Blotting, Western
MH  - Carbohydrates/chemistry
MH  - Cloning, Molecular
MH  - DNA/chemistry
MH  - DNA, Complementary/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epitopes/chemistry
MH  - Escherichia coli/metabolism
MH  - Esterases/metabolism
MH  - Glycosylation
MH  - Humans
MH  - Immunoglobulin E/chemistry
MH  - Isoelectric Focusing
MH  - Latex/*chemistry/metabolism
MH  - Lipase/metabolism
MH  - Medicago/metabolism
MH  - Medicago sativa/metabolism
MH  - Molecular Sequence Data
MH  - Peptides/chemistry
MH  - Plant Proteins
MH  - Protein Binding
MH  - Protein Sorting Signals
MH  - Protein Structure, Tertiary
MH  - Proteins/chemistry
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology, Amino Acid
MH  - Glycine max/metabolism
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Trypsin/pharmacology
EDAT- 2004/03/17 05:00
MHDA- 2004/07/09 05:00
CRDT- 2004/03/17 05:00
PHST- 2004/03/17 05:00 [pubmed]
PHST- 2004/07/09 05:00 [medline]
PHST- 2004/03/17 05:00 [entrez]
AID - S0021-9258(20)66522-X [pii]
AID - 10.1074/jbc.M309800200 [doi]
PST - ppublish
SO  - J Biol Chem. 2004 Jun 4;279(23):23933-41. doi: 10.1074/jbc.M309800200. Epub 2004 
      Mar 15.