PMID- 15026187
OWN - NLM
STAT- MEDLINE
DCOM- 20041021
LR  - 20061115
IS  - 0303-7207 (Print)
IS  - 0303-7207 (Linking)
VI  - 215
IP  - 1-2
DP  - 2004 Feb 27
TI  - Characterization of the adrenal-specific antigen IZA (inner zone antigen) and its 
      role in the steroidogenesis.
PG  - 143-8
AB  - Inner zone antigen (IZA) is a protein specifically expressed in the zona 
      fasciculata and reticularis of the adrenal cortex. The cDNA encoding IZA was 
      found to be identical to that encoding the previously reported putative 
      membrane-associated progesterone receptor (MPR) and the TCDD-induced 25kDa 
      protein (25-Dx). From its structure, MPR was classed as a member of a protein 
      family containing a haem-binding domain, and progesterone was proposed to be a 
      ligand of this domain. Indeed, when GST-tagged IZA was expressed in Escherichia 
      coli and purified, the purified GST-IZA had a brown colour with maximum 
      absorbance at 400 nm. The addition of dithionate shifted the absorbance peak to 
      420 nm, suggesting a haem-binding function. The possible role of IZA in 
      steroidogenesis has been addressed, and the inhibition of adrenal steroidogenesis 
      by the addition of an anti-IZA monoclonal antibody has been reported. When COS-7 
      cells were transformed with plasmids for appropriate steroidogenic enzymes in the 
      presence or absence of an IZA expression plasmid and tested for their 
      steroidogenic activities, 21-hydroxylation of progesterone was found to be 
      specifically activated by IZA overexpression, suggesting the involvement of IZA 
      in progesterone metabolism. Taken together, the available evidence suggests that 
      IZA may have an important role in the functions of the adrenal zona fasciculata 
      and reticularis.
FAU - Min, Li
AU  - Min L
AD  - Department of Molecular Physiological Chemistry, Graduate School of Medicine 
      (H-1), Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
FAU - Takemori, Hiroshi
AU  - Takemori H
FAU - Nonaka, Yasuki
AU  - Nonaka Y
FAU - Katoh, Yoshiko
AU  - Katoh Y
FAU - Doi, Junko
AU  - Doi J
FAU - Horike, Nanao
AU  - Horike N
FAU - Osamu, Hatano
AU  - Osamu H
FAU - Raza, Farah S
AU  - Raza FS
FAU - Vinson, Gavin P
AU  - Vinson GP
FAU - Okamoto, Mitshuhiro
AU  - Okamoto M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Adrenal Cortex Hormones)
RN  - 0 (Membrane Proteins)
RN  - 0 (PGRMC1 protein, human)
RN  - 0 (Receptors, Progesterone)
RN  - 0 (Receptors, Steroid)
SB  - IM
MH  - Adrenal Cortex/*metabolism
MH  - Adrenal Cortex Hormones/*metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Humans
MH  - Membrane Proteins/*metabolism
MH  - Molecular Sequence Data
MH  - Receptors, Progesterone/*metabolism
MH  - Receptors, Steroid/*metabolism
MH  - Sequence Homology, Amino Acid
MH  - Zona Fasciculata/cytology/*metabolism
MH  - Zona Reticularis/cytology/*metabolism
RF  - 20
EDAT- 2004/03/18 05:00
MHDA- 2004/10/22 09:00
CRDT- 2004/03/18 05:00
PHST- 2004/03/18 05:00 [pubmed]
PHST- 2004/10/22 09:00 [medline]
PHST- 2004/03/18 05:00 [entrez]
AID - S030372070300515X [pii]
AID - 10.1016/j.mce.2003.11.025 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2004 Feb 27;215(1-2):143-8. doi: 10.1016/j.mce.2003.11.025.