PMID- 15027182
OWN - NLM
STAT- MEDLINE
DCOM- 20040615
LR  - 20101118
IS  - 1009-3591 (Print)
IS  - 1009-3591 (Linking)
VI  - 10
IP  - 2
DP  - 2004 Feb
TI  - [The mechanism of tetragametic chimerism in a true hermaphroditism with 46, XX/46 
      ,XY].
PG  - 107-12
AB  - OBJECTIVE: To report a true hermaphroditism due to a teragametic chimerism and to 
      discuss the pathogenesis of tetragametic chimerism. METHODS: Chromosomal analysis 
      and fluorescence in situ hybridization(FISH) were carried out on the lymphocytes 
      from the blood and on the fibroblasts from the cultured skin and on fibroblasts 
      from two different kinds of gonadal tissues of the patient with ambiguous 
      genitalia respectively. Blood groups, human leukocyte antigen (HLA) haplotyping 
      and 77 short tandem repeat (STR) microsatellite markers were tested. The two 
      kinds of tissues in the gonad were detected by histopathological examination. 
      Blood groups, HLA haplotying and 77 STR microsatellite markers parents of the 
      patient's were also analyzed. RESULTS: Either 46,XX or 46,XY karyotype was found 
      in the lymphocytes of the blood and in the fibroblasts of the cultured skin and 
      of the two different kinds of gonadal tissues. Two X chromosome-specific signals 
      or one X and one Y signal were detected in each interphase nucleus by FISH from 
      the lymphocytes of the blood and the fibroblasts of three different tissue 
      cultures. The karyotype of the 46,XY cell line predominated in all cultures 
      except the cultured-fibroblasts from yellow gonadal tissues. STR marker analysis, 
      ABO grouping and HLA study from the patient were identified a single haplotype in 
      the patient from the mother and two different haplotypes from the father. Two 
      kinds of tissues in the gonad were observed by histopathological examination. The 
      yellow tissue was ovary and the white one was testis. CONCLUSIONS: 
      Histopathological examination and chromosomal analysis combined with FISH are 
      very useful methods for the diagnosis of true hermaphroditism. Blood typing, HLA 
      and short tandem repeat microsatellite markers afford strong evidence for 
      confirming tetragametic chimerism. The mechanism of tetragametic chimerism in 
      true hermaphroditism can be explained by a parthenogenetic division of a haploid 
      nucleu into two identical gametes, followed by fertilization with both X and Y 
      spermatozoa and then developed into an organism.
FAU - Cui, Yingxia
AU  - Cui Y
AD  - Laboratory of Reproduction and Genetics, Nanjing General Hospital, Nanjing 
      Command, PLA, Nanjing, Jiangsu 210002, China. cuiyx55@yahoo.com
FAU - Zhu, Peiyuan
AU  - Zhu P
FAU - Ye, Xiaoqin
AU  - Ye X
FAU - Wu, Yuanzhe
AU  - Wu Y
FAU - Wang, Yongmei
AU  - Wang Y
FAU - Yin, Honglin
AU  - Yin H
FAU - Yao, Bing
AU  - Yao B
FAU - Huang, Yufeng
AU  - Huang Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Nan Ke Xue
JT  - Zhonghua nan ke xue = National journal of andrology
JID - 101093592
RN  - 0 (ABO Blood-Group System)
SB  - IM
MH  - ABO Blood-Group System
MH  - *Chimera
MH  - Disorders of Sex Development/blood/*genetics/pathology
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - *Sex Chromosomes
EDAT- 2004/03/19 05:00
MHDA- 2004/06/16 05:00
CRDT- 2004/03/19 05:00
PHST- 2004/03/19 05:00 [pubmed]
PHST- 2004/06/16 05:00 [medline]
PHST- 2004/03/19 05:00 [entrez]
PST - ppublish
SO  - Zhonghua Nan Ke Xue. 2004 Feb;10(2):107-12.