PMID- 15027619
OWN - NLM
STAT- MEDLINE
DCOM- 20040928
LR  - 20161124
IS  - 1532-0820 (Print)
IS  - 1532-0820 (Linking)
VI  - 54
IP  - 1
DP  - 2004 Feb
TI  - Effects of nonylphenol and phytoestrogen-enriched diet on plasma vitellogenin, 
      steroid hormone, hepatic cytochrome P450 1A, and glutathione-S-transferase values 
      in goldfish (Carassius auratus).
PG  - 54-62
AB  - The effects of nonylphenol (NP) on plasma vitellogenin (VTG) and steroid hormone 
      values, as well as hepatic cytochrome P450 1A (CYP1A) and 
      glutathione-S-transferase (GST) activities, were measured in goldfish (Carassius 
      auratus) fed a diet with a low (formulated diet, FD) or high (commercial diet, 
      CD) content of phytoestrogens, including genistein and daidzein. Male goldfish 
      with secondary sexual characteristics were exposed to nominal NP concentrations 
      of 0.1, 1.0, 10, and 100 microg/L in the water for 28 days while being fed either 
      the FD or CD diet at 1.0% of body weight daily. Plasma VTG concentration in male 
      goldfish exposed to 100 microg of NP/L and fed FD was significantly higher than 
      that in the FD-fed control fish at seven, 21, and 28 days. However, fish of the 
      CD-fed group exposed to 100 microg of NP/ L had significantly higher plasma VTG 
      concentration than did fish of the CD-fed control group at 28 days only. 
      Moreover, plasma VTG concentration in fish of the CD-fed control group was about 
      100-fold higher than that in fish of the FD-fed control group. Although the 
      estrogenic effects of a phytoestrogen-enriched diet caused a decrease in 
      testosterone and/or 11-ketotestosterone values in the CD-fed fish, there was no 
      dose-response relationship between androgen and amount of NP to which the FD-fed 
      fish were exposed. Nonylphenol does not have appreciable effects on hepatic CYP1A 
      and GST activities in male goldfish at concentrations as low as 100 microg/L. 
      These results suggest that NP has estrogenic activity in male goldfish at the 
      nominal concentration of 100 microg/L, and that phytoestrogens, such as genistein 
      and daidzein, in the CD inhibit an aspect(s) of steroid release and/or synthesis 
      common to testosterone and 11-ketotestosterone. However, results of in vivo 
      screening assays for endocrine-disrupting chemicals may be seriously affected by 
      phytoestrogens in the diet, depending on content or potency of estrogenic 
      activity; therefore, we recommend use in research of a standardized, open-formula 
      diet in which estrogenic substances have been reduced to amounts that do not 
      alter the results of studies that are influenced by exogenous estrogens.
FAU - Ishibashi, Hiroshi
AU  - Ishibashi H
AD  - Faculty of Environmental and Symbiotic Sciences, Prefectural University of 
      Kumamoto, 3-1-100 Tsukide, Kumamoto 862-8502, Japan.
FAU - Tachibana, Katsuyasu
AU  - Tachibana K
FAU - Tsuchimoto, Mutsuyosi
AU  - Tsuchimoto M
FAU - Soyano, Kiyoshi
AU  - Soyano K
FAU - Tatarazako, Norihisa
AU  - Tatarazako N
FAU - Matsumura, Naomi
AU  - Matsumura N
FAU - Tomiyasu, Yuki
AU  - Tomiyasu Y
FAU - Tominaga, Nobuaki
AU  - Tominaga N
FAU - Arizono, Koji
AU  - Arizono K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Comp Med
JT  - Comparative medicine
JID - 100900466
RN  - 0 (Gonadal Steroid Hormones)
RN  - 0 (Isoflavones)
RN  - 0 (Phenols)
RN  - 0 (Phytoestrogens)
RN  - 0 (Plant Preparations)
RN  - 0 (Vitellogenins)
RN  - 79F6A2ILP5 (nonylphenol)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Animals
MH  - Cytochrome P-450 CYP1A1/metabolism
MH  - Diet
MH  - Dose-Response Relationship, Drug
MH  - Glutathione Transferase/metabolism
MH  - *Goldfish/blood
MH  - Gonadal Steroid Hormones/*blood
MH  - *Hepatopancreas/drug effects/enzymology
MH  - Isoflavones/*administration & dosage
MH  - Male
MH  - Phenols/*administration & dosage
MH  - Phytoestrogens
MH  - Plant Preparations/*administration & dosage
MH  - Vitellogenins/*blood
EDAT- 2004/03/19 05:00
MHDA- 2004/09/29 05:00
CRDT- 2004/03/19 05:00
PHST- 2004/03/19 05:00 [pubmed]
PHST- 2004/09/29 05:00 [medline]
PHST- 2004/03/19 05:00 [entrez]
PST - ppublish
SO  - Comp Med. 2004 Feb;54(1):54-62.