PMID- 15030410
OWN - NLM
STAT- MEDLINE
DCOM- 20040426
LR  - 20231213
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 89
IP  - 1
DP  - 2004 Apr
TI  - Exogenous, but not endogenous nociceptin modulates mesolimbic dopamine release in 
      mice.
PG  - 257-63
AB  - The effect of nociceptin (an endogenous ligand of the ORL1 receptor) on 
      mesolimbic dopamine release and simultaneous horizontal locomotion was studied in 
      freely moving mice undergoing microdialysis of the nucleus accumbens. 
      Intracerebroventricular (i.c.v.) administration of nociceptin (7 nmol) induced a 
      long-lasting suppression of mesolimbic dopamine release and horizontal locomotion 
      in wild-type but not ORL1 knockout mice. I.c.v. administration of the recently 
      reported peptide nociceptin antagonist [Nphe1, Arg14, Lys15] nociceptin-NH(2) 
      (known also as UFP-101, 5 nmol) completely abolished the suppressive effect of 
      nociceptin on mesolimbic dopamine release. However, UFP-101 administration alone 
      induced a mild and lasting suppression of mesolimbic dopamine release in both 
      wild-type and ORL1 knockout mice that was magnified in ORL1 knockout mice by 
      coadministration of nociceptin. UFP-101 administration alone suppressed 
      locomotion in both genotypes. These results confirm that the suppressive action 
      of nociceptin on mesolimbic dopamine release is mediated entirely by the ORL1 
      receptor, and that UFP-101 effectively antagonizes this action. However, the lack 
      of a stimulatory effect of UFP-101 in wild-type mice indicates that despite being 
      sensitive to exogenous nociceptin action, basal mesolimbic dopaminergic activity 
      is not determined by endogenous nociceptin in mice.
FAU - Koizumi, Miwako
AU  - Koizumi M
AD  - Neural Circuit Mechanisms Research Group, RIKEN Brain Science Institute, Saitama, 
      Japan.
FAU - Midorikawa, Naoko
AU  - Midorikawa N
FAU - Takeshima, Hiroshi
AU  - Takeshima H
FAU - Murphy, Niall P
AU  - Murphy NP
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 ((Nphe(1),Arg(14),Lys(15))N-OFQ NH(2))
RN  - 0 (Ligands)
RN  - 0 (Opioid Peptides)
RN  - 0 (Receptors, Opioid)
RN  - VTD58H1Z2X (Dopamine)
RN  - 0 (Nociceptin Receptor)
RN  - 0 (Oprl1 protein, mouse)
SB  - IM
MH  - Animals
MH  - Dopamine/*metabolism
MH  - Female
MH  - Injections, Intraventricular
MH  - Ligands
MH  - Male
MH  - Mice
MH  - Mice, Knockout
MH  - Microdialysis
MH  - Motor Activity/drug effects
MH  - Nucleus Accumbens/*drug effects/*metabolism
MH  - Opioid Peptides/administration & dosage/antagonists & 
      inhibitors/*metabolism/*pharmacology
MH  - Receptors, Opioid/genetics
MH  - Nociceptin Receptor
MH  - Nociceptin
EDAT- 2004/03/20 05:00
MHDA- 2004/04/27 05:00
CRDT- 2004/03/20 05:00
PHST- 2004/03/20 05:00 [pubmed]
PHST- 2004/04/27 05:00 [medline]
PHST- 2004/03/20 05:00 [entrez]
AID - JNC2322 [pii]
AID - 10.1111/j.1471-4159.2003.02322.x [doi]
PST - ppublish
SO  - J Neurochem. 2004 Apr;89(1):257-63. doi: 10.1111/j.1471-4159.2003.02322.x.