PMID- 15030878
OWN - NLM
STAT- MEDLINE
DCOM- 20041025
LR  - 20191210
IS  - 0731-7085 (Print)
IS  - 0731-7085 (Linking)
VI  - 35
IP  - 1
DP  - 2004 Apr 1
TI  - Development and validation of a high-performance liquid chromatographic method 
      for the determination of buspirone in pharmaceutical preparations.
PG  - 41-50
AB  - A stability indicating, reversed-phase high-performance liquid chromatographic 
      method was developed and validated for the determination of buspirone (Bsp) in 
      pharmaceutical dosage forms. The use of a semi-micro XTerra MS C18 (150 mm x 3.0 
      mm i.d., 5 microm particle size) analytical column, results in substantial 
      reduction in solvent consumption and increased sensitivity. The mobile phase 
      consisted of a mixture of 0.010 M ammonium acetate (pH 4.0) and methanol (55:45, 
      v/v), pumped at a flow rate 0.30 ml min-1. The UV detector was operated at 245 
      nm. The retention times for lidocaine (Ldc), which was used as internal standard, 
      and buspirone were 4.57 and 7.72 min, respectively. The calibration graph was 
      ranged from 1.00 to 5.00 microg ml-1, while detection and quantitation limits 
      were found to be 0.22 and 0.67 microg ml-1, respectively. The intra- and 
      inter-day relative standard deviation (% R.S.D.) values were less than 1.94%, 
      while the relative percentage error (% Er) was less than 4.0% (n = 5). The method 
      was applied to the quality control of commercial tablets and content uniformity 
      test and proved to be suitable for rapid and reliable quality control.
FAU - Zaxariou, M
AU  - Zaxariou M
AD  - Division of Pharmaceutical Chemistry, School of Pharmacy, University of Athens, 
      Panepistimiopolis, Zografou 157 71, Athens, Greece.
FAU - Panderi, I
AU  - Panderi I
LA  - eng
PT  - Journal Article
PT  - Validation Study
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Anti-Anxiety Agents)
RN  - 0 (Tablets)
RN  - TK65WKS8HL (Buspirone)
SB  - IM
MH  - Anti-Anxiety Agents/*analysis
MH  - Buspirone/*analysis
MH  - Calibration
MH  - Chromatography, High Pressure Liquid/instrumentation/*methods
MH  - Drug Stability
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Tablets
EDAT- 2004/03/20 05:00
MHDA- 2004/10/27 09:00
CRDT- 2004/03/20 05:00
PHST- 2003/08/06 00:00 [received]
PHST- 2003/12/15 00:00 [revised]
PHST- 2003/12/18 00:00 [accepted]
PHST- 2004/03/20 05:00 [pubmed]
PHST- 2004/10/27 09:00 [medline]
PHST- 2004/03/20 05:00 [entrez]
AID - S0731708503007295 [pii]
AID - 10.1016/j.jpba.2003.12.014 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2004 Apr 1;35(1):41-50. doi: 10.1016/j.jpba.2003.12.014.