PMID- 15032949
OWN - NLM
STAT- MEDLINE
DCOM- 20040618
LR  - 20191210
IS  - 0018-0661 (Print)
IS  - 0018-0661 (Linking)
VI  - 140
IP  - 1
DP  - 2004
TI  - Detection and physical mapping of the 18S-5.8S-26S rDNA and the pKFJ660 probe 
      with microsatellite sequences derived from the rice blast fungus (Magnaporthe 
      grisea) in conifer species.
PG  - 70-8
AB  - Sequences homologous to the pKFJ660 probe, a fragment of DNA derived from the 
      rice blast fungus (Magnaporthe grisea) carrying TC/AG repeat microsatellite 
      sequences and 30 bp direct repeats were identified in the genome of Picea 
      (spruce) and Pinus (pine) species by fluorescence in situ hybridization (FISH) 
      and slot blot analyses. Slot blot analysis using the pKFJ660 probe revealed 
      hybridization signals with genomic DNAs from various pine and spruce species. 
      Further analyses indicated that the copy number of the (AG)30 motif was higher 
      than 5 x 10(4) per plant genome for all plant samples tested, but the copy number 
      of the sequences homologous to the whole pKFJ660 probe varies considerably among 
      the 25 plant species tested. In situ hybridization of metaphase chromosomes from 
      Pinus resinosa, P. banksiana and P. strobus showed the presence of sequences 
      homologous to this probe on several chromosomes in a dispersed pattern. Major 
      signals were observed on a few chromosomes indicating that some of these 
      sequences are clustered in specific genomic locations. The locations of these 
      repeats were compared to those of 18S-5.8S-26S rDNA in pine species. Chromosomal 
      distribution of 18S-5.8S-26S rDNA varied among the three pine species (P. 
      resinosa, P. banksiana and P. strobus) studied. Ribosomal DNA (rDNA) sites were 
      identified on 14 to 20 chromosomes in these pine species.
FAU - Nkongolo, K K
AU  - Nkongolo KK
AD  - Department of Biological Sciences, Laurentian University, Sudbury, Ontario, 
      Canada. knkongolo@laurentian.ca
FAU - Kim, N S
AU  - Kim NS
FAU - Michael, P
AU  - Michael P
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Hereditas
JT  - Hereditas
JID - 0374654
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Fungal)
RN  - 0 (RNA, Ribosomal, 18S)
RN  - 0 (RNA, Ribosomal, 5.8S)
SB  - IM
MH  - Base Sequence
MH  - Chromosomes, Plant/metabolism
MH  - DNA Probes
MH  - DNA, Fungal
MH  - Genetic Variation
MH  - Genome, Plant
MH  - Hybridization, Genetic
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Magnaporthe/*genetics/pathogenicity
MH  - *Microsatellite Repeats
MH  - Molecular Sequence Data
MH  - *Physical Chromosome Mapping
MH  - Picea/genetics/microbiology
MH  - Pinus/genetics/microbiology
MH  - RNA, Ribosomal, 18S/*genetics
MH  - RNA, Ribosomal, 5.8S/*genetics
MH  - Species Specificity
MH  - Tracheophyta/*genetics/microbiology
EDAT- 2004/03/23 05:00
MHDA- 2004/06/23 05:00
CRDT- 2004/03/23 05:00
PHST- 2004/03/23 05:00 [pubmed]
PHST- 2004/06/23 05:00 [medline]
PHST- 2004/03/23 05:00 [entrez]
AID - hrd1691 [pii]
AID - 10.1111/j.1601-5223.2004.01691.x [doi]
PST - ppublish
SO  - Hereditas. 2004;140(1):70-8. doi: 10.1111/j.1601-5223.2004.01691.x.