PMID- 15034762
OWN - NLM
STAT- MEDLINE
DCOM- 20040713
LR  - 20071115
IS  - 0939-5555 (Print)
IS  - 0939-5555 (Linking)
VI  - 83
IP  - 6
DP  - 2004 Jun
TI  - Discrimination of chronic lymphocytic leukemia (CLL) and CLL/PL by cytomorphology 
      can clearly be correlated to specific genetic markers as investigated by 
      interphase fluorescence in situ hybridization (FISH).
PG  - 349-55
AB  - Although interphase fluorescence in situ hybridization (FISH) is routinely used 
      in chronic lymphocytic leukemia (CLL), differences in the chromosomal pattern 
      with respect to morphological subtypes of CLL (typical CLL, CLL/PL, PLL) are 
      still under debate. We studied 153 patients with CLL and correlated 
      cytomorphology on peripheral blood stains with FISH analysis and other prognostic 
      markers. The percentage of prolymphocytes was calculated as a continuous variable 
      and followed published thresholds in parallel while being correlated to FISH 
      analysis. Higher percentages of prolymphocytes were associated significantly with 
      deletion of 17p13. Deletion of 17p13 was most frequently observed in patients 
      with more than 30% prolymphocytes. Trisomy 12 was found mainly in cases with 
      6-30% prolymphocytes. The percentage of prolymphocytes did not correlate with 
      deletions of 11q23 or with 13q14 abnormalities. In conclusion, we suggest that 
      further research focus on the percentage of prolymphocytes in CLL. Doing so, 
      biologically relevant thresholds for the percentages of prolymphocytes in the 
      peripheral blood and their association to underlying genetic markers could be 
      investigated together with other biologically and especially prognostic markers.
FAU - Bacher, U
AU  - Bacher U
AD  - Laboratory for Leukemia Diagnostics, Department for Internal Medicine III, 
      Klinikum Grosshadern, Ludwig-Maximilians-University, Marchioninistr. 15, 81377, 
      Munich, Germany.
FAU - Kern, W
AU  - Kern W
FAU - Schoch, C
AU  - Schoch C
FAU - Hiddemann, W
AU  - Hiddemann W
FAU - Haferlach, T
AU  - Haferlach T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20040318
PL  - Germany
TA  - Ann Hematol
JT  - Annals of hematology
JID - 9107334
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosome Aberrations
MH  - Cohort Studies
MH  - Cytogenetics
MH  - Diagnosis, Differential
MH  - Female
MH  - Genetic Markers/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/blood/*diagnosis/genetics/pathology
MH  - Leukemia, Prolymphocytic/blood/*diagnosis/genetics/pathology
MH  - Logistic Models
MH  - Male
MH  - Middle Aged
MH  - Prospective Studies
EDAT- 2004/03/23 05:00
MHDA- 2004/07/14 05:00
CRDT- 2004/03/23 05:00
PHST- 2003/10/11 00:00 [received]
PHST- 2004/03/02 00:00 [accepted]
PHST- 2004/03/23 05:00 [pubmed]
PHST- 2004/07/14 05:00 [medline]
PHST- 2004/03/23 05:00 [entrez]
AID - 10.1007/s00277-004-0869-4 [doi]
PST - ppublish
SO  - Ann Hematol. 2004 Jun;83(6):349-55. doi: 10.1007/s00277-004-0869-4. Epub 2004 Mar 
      18.