PMID- 15036087
OWN - NLM
STAT- MEDLINE
DCOM- 20041026
LR  - 20131121
IS  - 1386-1425 (Print)
IS  - 1386-1425 (Linking)
VI  - 60
IP  - 4
DP  - 2004 Mar
TI  - Spectrophotometric and fluorimetric determination of diazepam, bromazepam and 
      clonazepam in pharmaceutical and urine samples.
PG  - 771-80
AB  - New spectrophotometric and fluorimetric methods have been developed to determine 
      diazepam, bromazepam and clonazepam (1,4-benzodiazepines) in pure forms, 
      pharmaceutical preparations and biological fluid. The new methods are based on 
      measuring absorption or emission spectra in methanolic potassium hydroxide 
      solution. Fluorimetric methods have proved selective with low detection limits, 
      whereas photometric methods showed relatively high detection limits. Successive 
      applications of developed methods for drugs determination in pharmaceutical 
      preparations and urine samples were performed. Photometric methods gave linear 
      calibration graphs in the ranges of 2.85-28.5, 0.316-3.16, and 0.316-3.16 
      microgml-1 with detection limits of 1.27, 0.08 and 0.13 microgml-1 for diazepam, 
      bromazepam and clonazepam, respectively. Corresponding average errors of 2.60, 
      5.26 and 3.93 and relative standard deviations (R.S.D.s) of 2.79, 2.12 and 2.83, 
      respectively, were obtained. Fluorimetric methods gave linear calibration graphs 
      in the ranges of 0.03-0.34, 0.03-0.32 and 0.03-0.38 microgml-1 with detection 
      limits of 7.13, 5.67 and 16.47 ngml-1 for diazepam, bromazepam and clonazepam, 
      respectively. Corresponding average errors of 0.29, 4.33 and 5.42 and R.S.D.s of 
      1.27, 1.96 and 1.14 were obtained, respectively. Statistical Students t-test and 
      F-test have been used and satisfactory results were obtained.
FAU - Salem, A A
AU  - Salem AA
AD  - Department of Chemistry, Faculty of Science, UAE University, Al-Ain, United Arab 
      Emirates. asalem@uaeu.ac.ae
FAU - Barsoum, B N
AU  - Barsoum BN
FAU - Izake, E L
AU  - Izake EL
LA  - eng
PT  - Journal Article
PL  - England
TA  - Spectrochim Acta A Mol Biomol Spectrosc
JT  - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
JID - 9602533
RN  - 0 (Anti-Anxiety Agents)
RN  - 0 (Anticonvulsants)
RN  - 0 (Hydroxides)
RN  - 0 (Pharmaceutical Preparations)
RN  - 0 (Potassium Compounds)
RN  - 5PE9FDE8GB (Clonazepam)
RN  - Q3JTX2Q7TU (Diazepam)
RN  - WZH3C48M4T (potassium hydroxide)
RN  - X015L14V0O (Bromazepam)
SB  - IM
MH  - Anti-Anxiety Agents/analysis/urine
MH  - Anticonvulsants/analysis/urine
MH  - Bromazepam/*analysis/urine
MH  - Calibration
MH  - Chromatography, High Pressure Liquid
MH  - Clonazepam/*analysis/urine
MH  - Diazepam/*analysis/urine
MH  - Humans
MH  - Hydroxides/chemistry
MH  - Light
MH  - Pharmaceutical Preparations/analysis
MH  - Potassium Compounds/chemistry
MH  - Spectrometry, Fluorescence/*methods
MH  - Spectrophotometry/*methods
MH  - Urine
EDAT- 2004/03/24 05:00
MHDA- 2004/10/27 09:00
CRDT- 2004/03/24 05:00
PHST- 2003/03/06 00:00 [received]
PHST- 2003/06/30 00:00 [revised]
PHST- 2003/07/01 00:00 [accepted]
PHST- 2004/03/24 05:00 [pubmed]
PHST- 2004/10/27 09:00 [medline]
PHST- 2004/03/24 05:00 [entrez]
AID - S1386142503003007 [pii]
AID - 10.1016/S1386-1425(03)00300-7 [doi]
PST - ppublish
SO  - Spectrochim Acta A Mol Biomol Spectrosc. 2004 Mar;60(4):771-80. doi: 
      10.1016/S1386-1425(03)00300-7.