PMID- 15036361
OWN - NLM
STAT- MEDLINE
DCOM- 20040428
LR  - 20061115
IS  - 0891-0618 (Print)
IS  - 0891-0618 (Linking)
VI  - 27
IP  - 1
DP  - 2004 Mar
TI  - Co-expression pattern of neuronal nitric oxide synthase and two variants of 
      choline acetyltransferase in myenteric neurons of porcine ileum.
PG  - 33-41
AB  - Cholinergic enteric neurons were demonstrated immunohistochemically so far by 
      using antibodies staining the common choline acetyltransferase (cChAT) in neurons 
      of the central nervous system. The results of staining in the enteric nervous 
      system of various species were, however, not satisfactory. We describe here 
      findings obtained with a newly raised antibody against a peripheral variant of 
      choline acetyltransferase (pChAT) in myenteric neurons of the pig small 
      intestine. Triple labelling for pChAT/cChAT/neuronal nitric oxide synthase (nNOS) 
      revealed 19.7% of 1664 neurons (within 40 ganglia) to be immunoreactive 
      exclusively for pChAT whereas 29.6% were positive for cChAT alone and 18.8% were 
      reactive only for nNOS. Colocalization of pChAT and cChAT was found in 22.4%, of 
      pChAT and nNOS in 8.1% and of cChAT and nNOS in 1.4%. All three markers were 
      simultaneously found in only 1 of 1664 neurons. To investigate the presence and 
      possible colocalization of the above markers within morphologically defined 
      neuron types, triple labelling of cChAT or nNOS with pChAT and a neurofilament 
      (NF) antibody pool was applied and the coexpression patterns of pChAT and cChAT 
      as well as of pChAT and nNOS in 120 neurons of each type were recorded. All type 
      I, II, IV and V neurons displayed immunoreactivity either for one or both 
      cholinergic markers. These neuron types were considered to be cholinergic. All 
      type VI neurons, a descending neuron population, were negative for cChAT but 
      positive for nNOS. However, 95% were immunoreactive for both pChAT and nNOS. The 
      physiological significance of the possible co-existence of acetylcholine and 
      nitric oxide within type VI neurons remains to be clarified. It is concluded that 
      the pChAT and cChAT antibodies used here recognize partly different populations 
      of enteric neurons in the pig. Thus, for total immunohistochemical 
      characterization of cholinergic enteric neurons both forms of choline 
      acetyltransferase have to be considered.
FAU - Brehmer, Axel
AU  - Brehmer A
AD  - Department of Anatomy I, University of Erlangen-Nuremberg, Krankenhausstr. 9, 
      D-91054 Erlangen, Germany. axel.brehmer@anatomie1.med.uni-erlangen.de
FAU - Schrodl, Falk
AU  - Schrodl F
FAU - Neuhuber, Winfried
AU  - Neuhuber W
FAU - Tooyama, Ikuo
AU  - Tooyama I
FAU - Kimura, Hiroshi
AU  - Kimura H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Chem Neuroanat
JT  - Journal of chemical neuroanatomy
JID - 8902615
RN  - 0 (Isoenzymes)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type I)
RN  - EC 2.3.1.6 (Choline O-Acetyltransferase)
SB  - IM
MH  - Animals
MH  - Choline O-Acetyltransferase/*biosynthesis
MH  - Female
MH  - Ileum/innervation/metabolism
MH  - Immunohistochemistry
MH  - Isoenzymes/*biosynthesis
MH  - Male
MH  - Myenteric Plexus/*metabolism
MH  - Neurons/*cytology/metabolism
MH  - Nitric Oxide Synthase/*biosynthesis
MH  - Nitric Oxide Synthase Type I
MH  - Swine
EDAT- 2004/03/24 05:00
MHDA- 2004/04/29 05:00
CRDT- 2004/03/24 05:00
PHST- 2003/03/14 00:00 [received]
PHST- 2003/08/11 00:00 [revised]
PHST- 2003/09/07 00:00 [accepted]
PHST- 2004/03/24 05:00 [pubmed]
PHST- 2004/04/29 05:00 [medline]
PHST- 2004/03/24 05:00 [entrez]
AID - S0891061803000966 [pii]
AID - 10.1016/j.jchemneu.2003.09.002 [doi]
PST - ppublish
SO  - J Chem Neuroanat. 2004 Mar;27(1):33-41. doi: 10.1016/j.jchemneu.2003.09.002.