PMID- 15036389
OWN - NLM
STAT- MEDLINE
DCOM- 20040720
LR  - 20131121
IS  - 0531-5565 (Print)
IS  - 0531-5565 (Linking)
VI  - 39
IP  - 3
DP  - 2004 Mar
TI  - Calorie restriction attenuates age-related alterations in the plasma membrane 
      antioxidant system in rat liver.
PG  - 297-304
AB  - Aging is associated with increased production of reactive oxygen species and 
      oxidation-induced damage to intracellular structures and membranes. Caloric 
      restriction (CR) is the only non-genetic method proven to extend lifespan in 
      mammals. Although the mechanisms of CR remain to be clearly elucidated, 
      reductions in oxidative stress have been shown to increase lifespan in several 
      model systems. Oxidative stress can be attenuated by CR. Mitochondria and plasma 
      membrane (PM) are normal sources of free radicals. The PM has a trans-membrane 
      redox system that provides electrons to recycle lipophilic antioxidants, such as 
      alpha-tocopherol and coenzyme Q (CoQ). The idea developed in this study is that 
      the PM is intimately involved in cellular physiology controlling the relationship 
      of the cell to its environment. PM is the key for protecting cellular integrity 
      during aging. Specifically, we have investigated age-related alterations and the 
      effects of CR in the trans-PM redox (antioxidant) system in rat liver. We found 
      that age-related declines in the ratio of CoQ(10)/CoQ(9) and alpha-tocopherol in 
      liver PM were attenuated by CR compared to those fed ad libitum (AL). 
      CoQ-dependent NAD(P)H dehydrogenases were increased in CR old rat liver PMs. As a 
      consequence, the liver PM of CR old rats was more resistant to oxidative 
      stress-induced lipid peroxidation than AL rats. Thus, our results suggest that CR 
      induces a higher capacity to oxidize NAD(P)H in the PM of old rat livers and as a 
      result, a higher resistance to oxidative stress-induced damage.
FAU - De Cabo, R
AU  - De Cabo R
AD  - Laboratory of Experimental Gerontology, National Institute on Aging, NIH, 
      Gerontology Research Center, Box 10, 5600 Nathan Shock Drive, Baltimore, MD 
      21224-6825, USA. decabora@grc.nia.nih.gov
FAU - Cabello, R
AU  - Cabello R
FAU - Rios, M
AU  - Rios M
FAU - Lopez-Lluch, G
AU  - Lopez-Lluch G
FAU - Ingram, D K
AU  - Ingram DK
FAU - Lane, M A
AU  - Lane MA
FAU - Navas, P
AU  - Navas P
LA  - eng
PT  - Journal Article
PL  - England
TA  - Exp Gerontol
JT  - Experimental gerontology
JID - 0047061
RN  - 0 (Antioxidants)
RN  - 1339-63-5 (Ubiquinone)
RN  - 53-59-8 (NADP)
RN  - H4N855PNZ1 (alpha-Tocopherol)
SB  - IM
MH  - Aging/*physiology
MH  - Animals
MH  - Antioxidants/metabolism
MH  - Blotting, Western/methods
MH  - *Caloric Restriction
MH  - Cell Membrane/*metabolism
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Lipid Peroxidation
MH  - Liver/*metabolism
MH  - Male
MH  - NADP/metabolism
MH  - Oxidation-Reduction
MH  - Rats
MH  - Rats, Inbred F344
MH  - Ubiquinone/analysis/metabolism
MH  - alpha-Tocopherol/analysis/metabolism
EDAT- 2004/03/24 05:00
MHDA- 2004/07/21 05:00
CRDT- 2004/03/24 05:00
PHST- 2003/10/02 00:00 [received]
PHST- 2003/12/09 00:00 [revised]
PHST- 2003/12/16 00:00 [accepted]
PHST- 2004/03/24 05:00 [pubmed]
PHST- 2004/07/21 05:00 [medline]
PHST- 2004/03/24 05:00 [entrez]
AID - S0531556503003383 [pii]
AID - 10.1016/j.exger.2003.12.003 [doi]
PST - ppublish
SO  - Exp Gerontol. 2004 Mar;39(3):297-304. doi: 10.1016/j.exger.2003.12.003.