PMID- 15036397
OWN - NLM
STAT- MEDLINE
DCOM- 20040720
LR  - 20211203
IS  - 0531-5565 (Print)
IS  - 1873-6815 (Electronic)
IS  - 0531-5565 (Linking)
VI  - 39
IP  - 3
DP  - 2004 Mar
TI  - Effects of aerobic exercise training on the protein kinase B (PKB)/mammalian 
      target of rapamycin (mTOR) signaling pathway in aged skeletal muscle.
PG  - 379-85
AB  - The protein kinase B (PKB)/mammalian target of rapamycin (mTOR) signaling pathway 
      is thought to play a critical role in the regulation of protein synthesis and 
      skeletal muscle mass. The purpose of the present study was to determine the 
      effects of voluntary wheel running on the PKB/mTOR signaling pathway in muscles 
      from aged mice (20-22 months). The total levels of mTOR were 65% higher in 
      gastrocnemius muscles from aged mice subjected to wheel running compared to aged 
      sedentary mice (p-0.002) PKB phosphorlation on Ser473 was 45% higher in 
      gastrocnemius muscles from aged mice subjected to wheel running compared to aged 
      sedentary mice (p=0.01) The total abundance of PKB was 50% higher in 
      gastrocnemius muscles from wheel running mice compared to aged controls 
      (p=0.005). Three months of wheel running did not alter the total amount of p70 
      S6K in gastrocnemius muscle. Protein synthesis, as assessed by 
      [(14)C]phenylalanine incorporation into protein was significantly higher in 
      soleus muscles from aged mice subjected to wheel running compared to aged 
      sedentary mice (p-0.001) These findings indicate the aerobic exercise training 
      may attenuate the age-related decline in protein synthesis, a process that 
      appears to be due, in part, to increases in mTOR and PKB.
FAU - Reynolds, Thomas H 4th
AU  - Reynolds TH 4th
AD  - Department of Internal Medicine, Division of Geriatric Medicine, University of 
      Michigan and the GRECC, Ann Arbor Veterans Affairs Healthcare System, Ann Arbor, 
      MI, USA. treynolds@ithaca.edu
FAU - Reid, Pamela
AU  - Reid P
FAU - Larkin, Lisa M
AU  - Larkin LM
FAU - Dengel, Donald R
AU  - Dengel DR
LA  - eng
GR  - K01 AG000701-04/AG/NIA NIH HHS/United States
GR  - T32 AG000114/AG/NIA NIH HHS/United States
GR  - K01-AG00710/AG/NIA NIH HHS/United States
GR  - T32-AG00114/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Exp Gerontol
JT  - Experimental gerontology
JID - 0047061
RN  - 0 (Muscle Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Aging/*physiology
MH  - Animals
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Muscle Contraction/physiology
MH  - Muscle Proteins/biosynthesis
MH  - Muscle, Skeletal/*metabolism
MH  - *Physical Conditioning, Animal
MH  - Protein Serine-Threonine Kinases/*metabolism
MH  - Proto-Oncogene Proteins/*metabolism
MH  - Proto-Oncogene Proteins c-akt
MH  - Signal Transduction/*physiology
MH  - Sirolimus/*metabolism
MH  - Specific Pathogen-Free Organisms
PMC - PMC2714731
MID - NIHMS116631
EDAT- 2004/03/24 05:00
MHDA- 2004/07/21 05:00
PMCR- 2009/07/23
CRDT- 2004/03/24 05:00
PHST- 2003/09/30 00:00 [received]
PHST- 2003/12/15 00:00 [revised]
PHST- 2003/12/16 00:00 [accepted]
PHST- 2004/03/24 05:00 [pubmed]
PHST- 2004/07/21 05:00 [medline]
PHST- 2004/03/24 05:00 [entrez]
PHST- 2009/07/23 00:00 [pmc-release]
AID - S0531556503003607 [pii]
AID - 10.1016/j.exger.2003.12.005 [doi]
PST - ppublish
SO  - Exp Gerontol. 2004 Mar;39(3):379-85. doi: 10.1016/j.exger.2003.12.005.