PMID- 15039344
OWN - NLM
STAT- MEDLINE
DCOM- 20040507
LR  - 20240411
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 72
IP  - 4
DP  - 2004 Apr
TI  - Purification and molecular cloning of and immunization with Ancylostoma 
      ceylanicum excretory-secretory protein 2, an immunoreactive protein produced by 
      adult hookworms.
PG  - 2203-13
AB  - Hookworms remain major agents of global morbidity, and vaccination against these 
      bloodfeeding parasites may be an attractive complement to conventional control 
      methods. Here we describe the cloning of Ancylostoma ceylanicum 
      excretory-secretory protein 2 (AceES-2), a novel immunoreactive protein produced 
      by adult worms. Native AceES-2 was purified from excretory-secretory (ES) 
      products by reverse-phase high-pressure liquid chromatography, subjected to 
      amino-terminal sequencing, and cloned from adult worm RNA by using reverse 
      transcription-PCR. The translated AceES-2 cDNA predicts that the mature protein 
      consists of 102 amino acids and has a molecular mass of 11.66 kDa. Western 
      immunoblot and enzyme-linked immunosorbent assay analyses demonstrated that 
      recombinant AceES-2 (rAceES-2) reacted strongly with antibodies from A. 
      ceylanicum-infected hamsters. Immunization of hamsters with native ES products 
      adsorbed to alum induced antibodies that recognized rAceES-2, while rAceES-2-alum 
      vaccination resulted in antibodies that reacted with a single protein band in ES 
      products that closely approximated the size predicted for the native molecule. 
      Infected hamsters that were passively immunized with hyperimmune rabbit 
      anti-rAceES-2 serum exhibited more rapid and complete recovery from anemia than 
      controls that received normal serum. Oral immunization with rAceES-2 was 
      associated with significantly reduced anemia upon challenge, an outcome similar 
      to the outcome observed in hamsters that were orally vaccinated with soluble 
      hookworm extract (the latter animals were also resistant to weight loss). These 
      data suggest that AceES-2 plays an important role in the host-parasite 
      interaction and that vaccination against this protein may represent a useful 
      strategy for controlling hookworm anemia.
FAU - Bungiro, Richard D Jr
AU  - Bungiro RD Jr
AD  - Child Health Research Center, Department of Pediatrics, Yale University School of 
      Medicine, New Haven, Connecticut 06520-8081, USA. richard.bungiro@yale.edu
FAU - Solis, Carolina V
AU  - Solis CV
FAU - Harrison, Lisa M
AU  - Harrison LM
FAU - Cappello, Michael
AU  - Cappello M
LA  - eng
GR  - F32 AI051042/AI/NIAID NIH HHS/United States
GR  - R01 AI047929/AI/NIAID NIH HHS/United States
GR  - F32 AI51042/AI/NIAID NIH HHS/United States
GR  - R01 AI47929/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Antibodies, Helminth)
RN  - 0 (Antigens, Helminth)
RN  - 0 (Helminth Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Vaccines)
SB  - IM
MH  - Administration, Oral
MH  - Amino Acid Sequence
MH  - Ancylostoma/genetics/*immunology
MH  - Ancylostomiasis/immunology/parasitology/*prevention & control
MH  - Animals
MH  - Antibodies, Helminth/blood
MH  - Antigens, Helminth/administration & dosage/genetics/immunology/isolation & 
      purification
MH  - Base Sequence
MH  - *Cloning, Molecular
MH  - Cricetinae
MH  - Helminth Proteins/administration & dosage/genetics/*immunology/*isolation & 
      purification
MH  - Immunization
MH  - Immunization, Passive
MH  - Injections, Subcutaneous
MH  - Mesocricetus
MH  - Molecular Sequence Data
MH  - Recombinant Proteins/administration & dosage/genetics/metabolism
MH  - Sequence Analysis, DNA
MH  - Vaccines/*administration & dosage/genetics/immunology
PMC - PMC375217
EDAT- 2004/03/25 05:00
MHDA- 2004/05/08 05:00
PMCR- 2004/04/01
CRDT- 2004/03/25 05:00
PHST- 2004/03/25 05:00 [pubmed]
PHST- 2004/05/08 05:00 [medline]
PHST- 2004/03/25 05:00 [entrez]
PHST- 2004/04/01 00:00 [pmc-release]
AID - 1737 [pii]
AID - 10.1128/IAI.72.4.2203-2213.2004 [doi]
PST - ppublish
SO  - Infect Immun. 2004 Apr;72(4):2203-13. doi: 10.1128/IAI.72.4.2203-2213.2004.