PMID- 15041230
OWN - NLM
STAT- MEDLINE
DCOM- 20040430
LR  - 20131121
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 150
IP  - 1
DP  - 2004 Apr 1
TI  - A fluorescence in situ hybridization study of complex t(9;22) in two chronic 
      myelocytic leukemia cases with a masked Philadelphia chromosome.
PG  - 81-5
AB  - The t(9;22)(q34;q11) is evident in more than 90% of patients with chronic 
      myelocytic leukemia (CML) and gives rise to the Philadelphia chromosome (Ph). 
      Approximately 5%-10% of CML patients show variant translocations involving other 
      chromosomes in addition to chromosomes 9 and 22. In some variant translocations, 
      additional material is transferred on der(22), resulting in a masked Ph 
      chromosome. In this paper, we report two apparently Ph-negative (Ph-) CML cases 
      showing a t(7;9;22)(q22;q34;q11) and a t(8;9;22)(q12;q34;q11), respectively. A 
      detailed molecular cytogenetic characterization was performed by fluorescence in 
      situ hybridization (FISH), which disclosed the presence of the 5'BCR/3'ABL fusion 
      gene on the der(7) and der(8) chromosomes, respectively. Derivative (22) appeared 
      as a masked Ph chromosome in both cases. FISH analysis with appropriate BAC/PAC 
      clones allowed us to precisely characterize the complex chromosomal 
      rearrangements that were not detected by conventional cytogenetic analysis.
FAU - Zagaria, Antonella
AU  - Zagaria A
AD  - Department of Hematology, University of Bari, Piazza Giulio Cesare 11, 70124 
      Bari, Italy.
FAU - Anelli, Luisa
AU  - Anelli L
FAU - Albano, Francesco
AU  - Albano F
FAU - Storlazzi, Clelia Tiziana
AU  - Storlazzi CT
FAU - Liso, Arcangelo
AU  - Liso A
FAU - Roberti, Maria Grazia
AU  - Roberti MG
FAU - Buquicchio, Caterina
AU  - Buquicchio C
FAU - Liso, Vincenzo
AU  - Liso V
FAU - Rocchi, Mariano
AU  - Rocchi M
FAU - Specchia, Giorgina
AU  - Specchia G
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Chromosomes, Human, Pair 22/*genetics
MH  - Chromosomes, Human, Pair 8/genetics
MH  - Chromosomes, Human, Pair 9/*genetics
MH  - Fusion Proteins, bcr-abl/genetics/metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics
MH  - Leukemia, Myeloid, Chronic, Atypical, BCR-ABL Negative/*genetics
MH  - Male
MH  - Middle Aged
MH  - *Translocation, Genetic
EDAT- 2004/03/26 05:00
MHDA- 2004/05/01 05:00
CRDT- 2004/03/26 05:00
PHST- 2003/08/07 00:00 [received]
PHST- 2003/08/22 00:00 [accepted]
PHST- 2004/03/26 05:00 [pubmed]
PHST- 2004/05/01 05:00 [medline]
PHST- 2004/03/26 05:00 [entrez]
AID - S0165460803003741 [pii]
AID - 10.1016/j.cancergencyto.2003.08.018 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2004 Apr 1;150(1):81-5. doi: 
      10.1016/j.cancergencyto.2003.08.018.