PMID- 15041592
OWN - NLM
STAT- MEDLINE
DCOM- 20040419
LR  - 20210902
IS  - 0003-2999 (Print)
IS  - 0003-2999 (Linking)
VI  - 98
IP  - 4
DP  - 2004 Apr
TI  - The effect of local anesthetics on monocyte mCD14 and human leukocyte antigen-DR 
      expression.
PG  - 1024-1029
LID - 10.1213/01.ANE.0000104480.04856.93 [doi]
AB  - It has been demonstrated that local anesthetics have several effects on the 
      immune system. Monocytes and macrophages are essential components of the host 
      response to microbial infection; however, the effect of local anesthetics on 
      monocyte surface receptor expression remains unclear. We designed this study to 
      investigate the effects of local anesthetics on monocyte mCD14 and human 
      leukocyte antigen (HLA)-DR expression and lipopolysaccharide (LPS)-induced or 
      staphylococcal enterotoxin B (SEB)-induced tumor necrosis factor (TNF)-alpha 
      production. Blood samples were obtained from 10 healthy volunteers. The effects 
      of local anesthetics on LPS- or SEB-induced TNF-alpha production were determined 
      by using an enzyme-linked immunosorbent assay. After different doses of local 
      anesthetics were added, the blood was stimulated with LPS (10 ng/mL) or SEB (10 
      micro g/mL) for 4 h. The effects of local anesthetics on monocyte mCD14 and 
      HLA-DR expression were measured by dual monoclonal antibody staining and flow 
      cytometry. Local anesthetics showed no effect on LPS- or SEB-induced TNF-alpha 
      production in human whole blood. Local anesthetics suppressed monocyte HLA-DR 
      expression in a dose-dependent manner (P < 0.05) but had no effect on monocyte 
      mCD14 expression. This study demonstrated that local anesthetics suppress HLA-DR 
      expression on the surface of human monocytes. IMPLICATIONS: Monocyte surface 
      receptors have a crucial role in the host response to microbial infection. We 
      investigated the effects of local anesthetics on monocyte mCD14 and human 
      leukocyte antigen (HLA)-DR expression. Our results show that local anesthetics 
      suppress HLA-DR expression on the surface of human monocytes.
FAU - Kawasaki, Takashi
AU  - Kawasaki T
AD  - Department of Anesthesiology, University of Occupational and Environmental 
      Health, Kitakyushu, Japan.
FAU - Kawasaki, Chika
AU  - Kawasaki C
FAU - Ogata, Masanori
AU  - Ogata M
FAU - Shigematsu, Akio
AU  - Shigematsu A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Anesth Analg
JT  - Anesthesia and analgesia
JID - 1310650
RN  - 0 (Anesthetics, Local)
RN  - 0 (Enterotoxins)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 98PI200987 (Lidocaine)
RN  - B6E06QE59J (Mepivacaine)
RN  - Y8335394RO (Bupivacaine)
SB  - IM
MH  - Adult
MH  - Anesthetics, Local/*pharmacology
MH  - Bupivacaine/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Enterotoxins/pharmacology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - HLA-DR Antigens/*biosynthesis
MH  - Humans
MH  - In Vitro Techniques
MH  - Lidocaine/pharmacology
MH  - Lipopolysaccharide Receptors/*biosynthesis
MH  - Lipopolysaccharides/pharmacology
MH  - Male
MH  - Mepivacaine/pharmacology
MH  - Monocytes/drug effects/*metabolism
MH  - Tumor Necrosis Factor-alpha/biosynthesis
EDAT- 2004/03/26 05:00
MHDA- 2004/04/20 05:00
CRDT- 2004/03/26 05:00
PHST- 2004/03/26 05:00 [pubmed]
PHST- 2004/04/20 05:00 [medline]
PHST- 2004/03/26 05:00 [entrez]
AID - 00000539-200404000-00027 [pii]
AID - 10.1213/01.ANE.0000104480.04856.93 [doi]
PST - ppublish
SO  - Anesth Analg. 2004 Apr;98(4):1024-1029. doi: 10.1213/01.ANE.0000104480.04856.93.