PMID- 15044249
OWN - NLM
STAT- MEDLINE
DCOM- 20040817
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 104
IP  - 2
DP  - 2004 Jul 15
TI  - Rapid chemokine secretion from endothelial cells originates from 2 distinct 
      compartments.
PG  - 314-20
AB  - The neutrophil-attracting chemokine interleukin 8 (IL-8) is stored in the 
      Weibel-Palade body (WPB) of endothelial cells (ECs) from which it can be rapidly 
      released after exposure to the secretagogues histamine or thrombin. In this 
      manner, IL-8 may enable rapid recruitment of leukocytes to inflammatory sites. To 
      explore the possible storage of EC-derived chemokines that may attract other 
      subsets of leukocytes, we examined the intracellular localization and 
      secretagogue responsiveness of growth-related oncogene alpha (GROalpha), monocyte 
      chemoattractant protein-1 (MCP-1), eotaxin-3, interferon-gamma-inducible protein 
      10 (IP-10), and regulated on activation, normal T-cell expressed and secreted 
      (RANTES). While eotaxin-3, GROalpha, and MCP-1 were rapidly released from ECs, 
      the release of the T-cell attractors RANTES and IP-10 was not sensitive to the 
      secretagogues. Moreover, of the 3 former chemokines, only eotaxin-3 was stored in 
      WPBs. GROalpha and MCP-1 resided mainly in smaller vesicles compatible with 
      sorting to a different, histamine-responsive compartment, which has been 
      described in ECs although not reported to contain chemokines. In conclusion, we 
      propose that rapid release of chemokines is restricted to those primarily 
      recruiting leukocytes of the innate immune system, and that their storage in ECs 
      is not restricted to the WPB compartment.
FAU - Oynebraten, Inger
AU  - Oynebraten I
AD  - LIIPAT, Institute of Pathology, Rikshospitalet, N-0027 Oslo, Norway. 
      inger.oynebraten@labmed.uio.no
FAU - Bakke, Oddmund
AU  - Bakke O
FAU - Brandtzaeg, Per
AU  - Brandtzaeg P
FAU - Johansen, Finn-Eirik
AU  - Johansen FE
FAU - Haraldsen, Guttorm
AU  - Haraldsen G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040325
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (CCL26 protein, human)
RN  - 0 (CXCL1 protein, human)
RN  - 0 (Carcinogens)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL26)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokine CXCL1)
RN  - 0 (Chemokine CXCL10)
RN  - 0 (Chemokines)
RN  - 0 (Chemokines, CC)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Interleukin-8)
RN  - 0 (von Willebrand Factor)
RN  - 820484N8I3 (Histamine)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Carcinogens/pharmacology
MH  - Cell Compartmentation/*physiology
MH  - Cells, Cultured
MH  - Chemokine CCL2/metabolism
MH  - Chemokine CCL26
MH  - Chemokine CCL5/metabolism
MH  - Chemokine CXCL1
MH  - Chemokine CXCL10/metabolism
MH  - Chemokines/*metabolism
MH  - Chemokines, CC/metabolism
MH  - Chemokines, CXC/metabolism
MH  - Cytoplasm/metabolism
MH  - Endothelium, Vascular/cytology/drug effects/*metabolism
MH  - Histamine/pharmacology
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins/metabolism
MH  - Interleukin-8/metabolism
MH  - Secretory Vesicles/drug effects/metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Umbilical Veins/cytology
MH  - Weibel-Palade Bodies/metabolism
MH  - von Willebrand Factor/metabolism
EDAT- 2004/03/27 05:00
MHDA- 2004/08/18 05:00
CRDT- 2004/03/27 05:00
PHST- 2004/03/27 05:00 [pubmed]
PHST- 2004/08/18 05:00 [medline]
PHST- 2004/03/27 05:00 [entrez]
AID - S0006-4971(20)55122-7 [pii]
AID - 10.1182/blood-2003-08-2891 [doi]
PST - ppublish
SO  - Blood. 2004 Jul 15;104(2):314-20. doi: 10.1182/blood-2003-08-2891. Epub 2004 Mar 
      25.