PMID- 15044917
OWN - NLM
STAT- MEDLINE
DCOM- 20041014
LR  - 20230210
IS  - 0893-3952 (Print)
IS  - 0893-3952 (Linking)
VI  - 17
IP  - 6
DP  - 2004 Jun
TI  - Reliable detection of macrolide-resistant Helicobacter pylori via fluorescence in 
      situ hybridization in formalin-fixed tissue.
PG  - 684-9
AB  - Macrolide-resistant Helicobacter (H.) pylori represent an increasing therapeutic 
      problem. Macrolide resistance is usually determined phenotypically in vitro with 
      methods such as E-test or agar dilution test. A prerequisite for those tests, 
      however, is bacterial culture that is not routinely set up in the course of 
      gastroscopy. In contrast, formalin-fixed, paraffin-embedded biopsies are 
      regularly available from patients who have undergone gastroscopy. In such 
      biopsies macrolide-resistant H. pylori can be detected by the genotype-based 
      technique of fluorescence in situ hybridization (FISH). Experience gained by this 
      new method, however, is still extremely limited, especially in formalin-fixed 
      tissue. Therefore, we retrospectively investigated formalin-fixed, 
      paraffin-embedded biopsy specimens by FISH in 104 patients suffering from 
      therapy-resistant H. pylori gastritis. To test the accuracy of FISH, we initially 
      examined specimens from 53 patients for whom results of the E-test were 
      available. Next we analyzed biopsies from another 51 patients that had been 
      selected since phenotypical resistance testing had failed despite documented 
      culturing attempts. In all 104 patients, H. pylori was detected by FISH and could 
      thus be investigated for macrolide resistance. Overall, macrolide-resistant 
      bacteria were found in 71 patients (68.3%). In 49 of 53 patients (92.4%), FISH 
      and E-test returned identical results (no significant discordance according to 
      McNemar's chi(2)-test). Taken together, our study demonstrates that FISH is a 
      highly sensitive and reliable method for detecting macrolide-resistant H. pylori 
      in formalin-fixed, paraffin-embedded biopsy specimens, which represents the 
      routine method of processing tissue obtained upon gastroscopy.
FAU - Juttner, Stefan
AU  - Juttner S
AD  - Institute for Pathology, Klinikum Bayreuth, Bayreuth, Germany.
FAU - Vieth, Michael
AU  - Vieth M
FAU - Miehlke, Stephan
AU  - Miehlke S
FAU - Schneider-Brachert, Wulf
AU  - Schneider-Brachert W
FAU - Kirsch, Christian
AU  - Kirsch C
FAU - Pfeuffer, Thilo
AU  - Pfeuffer T
FAU - Lehn, Norbert
AU  - Lehn N
FAU - Stolte, Manfred
AU  - Stolte M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Mod Pathol
JT  - Modern pathology : an official journal of the United States and Canadian Academy 
      of Pathology, Inc
JID - 8806605
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Carbocyanines)
RN  - 0 (Macrolides)
RN  - 0 (cyanine dye 3)
RN  - 1HG84L3525 (Formaldehyde)
RN  - H1250JIK0A (Clarithromycin)
RN  - TPY09G7XIR (Fluorescein)
SB  - IM
MH  - Anti-Bacterial Agents/pharmacology
MH  - Carbocyanines/chemistry
MH  - Clarithromycin/pharmacology
MH  - Drug Resistance, Bacterial/genetics
MH  - Fluorescein/chemistry
MH  - Formaldehyde
MH  - Gastritis/diagnosis/microbiology
MH  - Helicobacter Infections/diagnosis/microbiology
MH  - Helicobacter pylori/*chemistry/drug effects/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Macrolides/pharmacology
MH  - Reproducibility of Results
MH  - Stomach/*microbiology/pathology
MH  - Tissue Fixation
EDAT- 2004/03/27 05:00
MHDA- 2004/10/16 09:00
CRDT- 2004/03/27 05:00
PHST- 2004/03/27 05:00 [pubmed]
PHST- 2004/10/16 09:00 [medline]
PHST- 2004/03/27 05:00 [entrez]
AID - S0893-3952(22)04359-9 [pii]
AID - 10.1038/modpathol.3800098 [doi]
PST - ppublish
SO  - Mod Pathol. 2004 Jun;17(6):684-9. doi: 10.1038/modpathol.3800098.