PMID- 15045586
OWN - NLM
STAT- MEDLINE
DCOM- 20040720
LR  - 20181113
IS  - 0945-6317 (Print)
IS  - 0945-6317 (Linking)
VI  - 444
IP  - 5
DP  - 2004 May
TI  - Immunohistochemical analysis of pro- and active-caspase 3 in laryngeal squamous 
      cell carcinoma.
PG  - 439-46
AB  - Active caspase 3 is considered to be the main executioner caspase in apoptotic 
      process. The mechanisms of apoptosis in laryngeal squamous cell carcinoma (LSCC) 
      have been investigated by examining the expression profiles of pro-caspase 3 and 
      active-caspase 3. The correlation between the two forms of caspase 3 and the p53 
      status was also determined. LSCCs ( n=65) were studied using immunohistochemistry 
      with antibodies to pro-caspase 3, active-caspase 3 and p53. The expression of 
      pro-caspase 3 was absent or weak in 16 (24.6%), moderate in 21 (32.3%) and strong 
      in 28 (43.1%) cases. Survival curves for different levels of pro-caspase 3 
      differed, but the differences were not statistically significant. An apoptotic 
      index (AI) was determined by quantifying the active-caspase 3-positive cells. The 
      AI ranged from 0.2% to 9.4% and did not differ among the different levels of 
      pro-caspase 3 expression. Even in cases in which the expression of pro-caspase 3 
      was considered negative, caspase 3-positive apoptotic cells were found. The AIs 
      were significantly higher in supraglottic tumours compared with glottic 
      counterparts ( P=0.008) and were higher in poorly differentiated tumours compared 
      with well-differentiated and moderately differentiated LSCC ( P=0.06). No 
      correlation between AI and p53 expression was found, although pro-caspase 3 
      expression trended to be higher in the p53-positive group of LSCC. Our results 
      suggest that the expression of pro-caspase 3, a key executioner caspase in 
      apoptosis, is downregulated in a proportion of LSCC, but this is not associated 
      with decreased apoptotic activity, measured by active-caspase 3 labelling.
FAU - Cor, Andrej
AU  - Cor A
AD  - Institute for Histology and Embryology, Medical Faculty of Ljubljana, Korytkova 
      2, 1000, Ljubljana, Slovenia, andrej.coer@mf.uni-lj.si
FAU - Pizem, Joze
AU  - Pizem J
FAU - Gale, Nina
AU  - Gale N
LA  - eng
PT  - Journal Article
DEP - 20040325
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Enzyme Precursors)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - EC 3.4.22.- (CASP3 protein, human)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Apoptosis
MH  - Biomarkers, Tumor/analysis
MH  - Carcinoma, Squamous Cell/*enzymology/mortality/pathology
MH  - Caspase 3
MH  - Caspases/*metabolism
MH  - Cell Count
MH  - Enzyme Precursors/*metabolism
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Laryngeal Neoplasms/*enzymology/mortality/pathology
MH  - Male
MH  - Middle Aged
MH  - Survival Rate
MH  - Tumor Suppressor Protein p53/analysis
EDAT- 2004/03/27 05:00
MHDA- 2004/07/21 05:00
CRDT- 2004/03/27 05:00
PHST- 2003/10/28 00:00 [received]
PHST- 2004/01/15 00:00 [accepted]
PHST- 2004/03/27 05:00 [pubmed]
PHST- 2004/07/21 05:00 [medline]
PHST- 2004/03/27 05:00 [entrez]
AID - 10.1007/s00428-004-0997-1 [doi]
PST - ppublish
SO  - Virchows Arch. 2004 May;444(5):439-46. doi: 10.1007/s00428-004-0997-1. Epub 2004 
      Mar 25.