PMID- 1504739
OWN - NLM
STAT- MEDLINE
DCOM- 19920924
LR  - 20190509
IS  - 0007-1188 (Print)
IS  - 0007-1188 (Linking)
VI  - 106
IP  - 3
DP  - 1992 Jul
TI  - Receptor-coupled shortening of alpha-toxin-permeabilized single smooth muscle 
      cells from the guinea-pig stomach.
PG  - 539-43
AB  - 1. Isolated single smooth muscle cells from the fundus of the guinea-pig stomach 
      were permeabilized by use of Staphylococcus aureus alpha-toxin. Receptor-coupled 
      shortening of individual cells was monitored under phase contrast microscopy. 2. 
      Most of the isolated cells responded to 0.6 microM Ca2+, but not to 0.3 microM 
      Ca2+, with a resulting maximal shortening to approximately 65% of the resting 
      cell length. The contractile activity of these permeabilized cells lasted for 
      several hours and repeated shortening was readily achieved after washing out. 3. 
      Addition of acetylcholine (ACh) at a maximal concentration (10 microM) resulted 
      in a marked decrease in the concentration of Ca2+ required to trigger a threshold 
      response from 0.6 microM to 0.2 microM, and 1 mM guanosine 5'-diphosphate (GDP) 
      blocked this decrease. Moreover, treatment with 100 microM guanosine 
      5'-triphosphate (GTP) mimicked the action of ACh. 4. Addition of 100 microM 
      inositol 1,4,5-trisphosphate (InsP3) with 0.2 microM Ca2+ did not cause cell 
      shortening, whereas 10 microM ACh with 0.2 microM Ca2+ did, suggesting that 
      InsP3-induced Ca2+ release is not involved in ACh-operated cell shortening. 5. 
      The present study demonstrates an alpha-toxin-permeabilized single smooth muscle 
      cell preparation which retains its receptor function and also provides an insight 
      into mechanisms leading to augmentation of Ca2+ sensitivity by stimulation of 
      muscarinic receptors or GTP-binding proteins.
FAU - Ono, T
AU  - Ono T
AD  - Department of Molecular Pharmacology, Meiji College of Pharmacy, Tokyo, Japan.
FAU - Mita, M
AU  - Mita M
FAU - Suga, O
AU  - Suga O
FAU - Hashimoto, T
AU  - Hashimoto T
FAU - Oishi, K
AU  - Oishi K
FAU - Uchida, M K
AU  - Uchida MK
LA  - eng
PT  - Journal Article
PL  - England
TA  - Br J Pharmacol
JT  - British journal of pharmacology
JID - 7502536
RN  - 0 (Receptors, Muscarinic)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - N9YNS0M02X (Acetylcholine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Acetylcholine/pharmacology
MH  - Animals
MH  - Calcium/pharmacology
MH  - Cell Membrane Permeability/*drug effects
MH  - Guinea Pigs
MH  - In Vitro Techniques
MH  - Muscle Contraction
MH  - Muscle, Smooth/cytology/*physiology
MH  - Receptors, Muscarinic/*physiology
MH  - Staphylococcus aureus
MH  - Stomach/cytology
MH  - Type C Phospholipases/*pharmacology
PMC - PMC1907580
EDAT- 1992/07/01 00:00
MHDA- 1992/07/01 00:01
PMCR- 1993/07/01
CRDT- 1992/07/01 00:00
PHST- 1992/07/01 00:00 [pubmed]
PHST- 1992/07/01 00:01 [medline]
PHST- 1992/07/01 00:00 [entrez]
PHST- 1993/07/01 00:00 [pmc-release]
AID - 10.1111/j.1476-5381.1992.tb14371.x [doi]
PST - ppublish
SO  - Br J Pharmacol. 1992 Jul;106(3):539-43. doi: 10.1111/j.1476-5381.1992.tb14371.x.