PMID- 15047568
OWN - NLM
STAT- MEDLINE
DCOM- 20040708
LR  - 20200930
IS  - 1040-0605 (Print)
IS  - 1040-0605 (Linking)
VI  - 287
IP  - 1
DP  - 2004 Jul
TI  - Expression of genes for B7-H3 and other T cell ligands by nasal epithelial cells 
      during differentiation and activation.
PG  - L217-25
AB  - Epithelial cells of the human respiratory tract express human leukocyte antigen 
      (HLA) and the costimulatory molecules B7-1 and B7-2. Little is known, however, 
      about the constitutive expression of genes encoding for the more recently 
      identified members of the B7 homolog family of costimulatory molecules or about 
      the influence of cellular differentiation and cytokines on their activity or on 
      that of HLA or B7-1 and B7-2. Human nasal epithelial (HNE) cells were grown at 
      the air-liquid interface (ALI) for 2 or 21 days to model in vivo conditions. 
      Expression of genes for HLA-B and HLA-DR1 increased during mucociliary 
      differentiation during this period and became more similar to HNE cells obtained 
      fresh by brush biopsy from nasal turbinates. Gene transcripts for B7-H3 and B7-H2 
      were abundantly expressed in cells cultured at the ALI, but neither their 
      activities nor that of B7-2 was significantly altered during differentiation. 
      IFN-gamma and TNF-alpha upregulated mRNA encoding for both HLA molecules, but not 
      for the B7 molecules. This study describes, for the first time, the expression of 
      B7-H3 and B7-H2 by HNE cells and thus expands the range of potential 
      costimulatory signals through which these cells may interact with activated 
      mucosal T lymphocytes. In addition, the results suggest that the extent of 
      mucociliary differentiation of cultured cells may influence this capability.
FAU - Saatian, Bahman
AU  - Saatian B
AD  - Department of Environmental Health Sciences, Johns Hopkins Bloomberg School of 
      Public Health, Baltimore, MD 21205, USA.
FAU - Yu, Xiao-Ying
AU  - Yu XY
FAU - Lane, Andrew P
AU  - Lane AP
FAU - Doyle, Thanh
AU  - Doyle T
FAU - Casolaro, Vincenzo
AU  - Casolaro V
FAU - Spannhake, Ernst Wm
AU  - Spannhake EW
LA  - eng
GR  - ES-03819/ES/NIEHS NIH HHS/United States
GR  - ES-09004/ES/NIEHS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20040326
PL  - United States
TA  - Am J Physiol Lung Cell Mol Physiol
JT  - American journal of physiology. Lung cellular and molecular physiology
JID - 100901229
RN  - 0 (Antigens, CD)
RN  - 0 (Carrier Proteins)
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-DR1 Antigen)
RN  - 0 (ICOSLG protein, human)
RN  - 0 (Inducible T-Cell Co-Stimulator Ligand)
RN  - 0 (Ligands)
RN  - 0 (Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
EIN - Am J Physiol Lung Cell Mol Physiol. 2005 Apr;288(4):L771. Yu, XiaoYing [corrected 
      to Yu, Xiao-Ying]
MH  - Antigens, CD
MH  - Biopsy/methods
MH  - Carrier Proteins/genetics/*metabolism
MH  - Cell Differentiation/genetics
MH  - Cells, Cultured
MH  - Female
MH  - Gene Expression/drug effects
MH  - HLA-B Antigens/genetics
MH  - HLA-DR1 Antigen/genetics
MH  - Humans
MH  - Inducible T-Cell Co-Stimulator Ligand
MH  - Interferon-gamma/pharmacology
MH  - Ligands
MH  - Male
MH  - Nasal Mucosa/*metabolism/*pathology
MH  - *Proteins
MH  - T-Lymphocytes/*metabolism
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2004/03/30 05:00
MHDA- 2004/07/09 05:00
CRDT- 2004/03/30 05:00
PHST- 2004/03/30 05:00 [pubmed]
PHST- 2004/07/09 05:00 [medline]
PHST- 2004/03/30 05:00 [entrez]
AID - 00132.2003 [pii]
AID - 10.1152/ajplung.00132.2003 [doi]
PST - ppublish
SO  - Am J Physiol Lung Cell Mol Physiol. 2004 Jul;287(1):L217-25. doi: 
      10.1152/ajplung.00132.2003. Epub 2004 Mar 26.