PMID- 15048083
OWN - NLM
STAT- MEDLINE
DCOM- 20040608
LR  - 20141120
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 23
IP  - 22
DP  - 2004 May 13
TI  - Methylation of the asparagine synthetase promoter in human leukemic cell lines is 
      associated with a specific methyl binding protein.
PG  - 3953-61
AB  - We have examined the methylation profiles of the asparagine synthetase (ASY) 
      promoter in a number of human leukemic cell lines in relation to their asparagine 
      (ASN) requirements in vitro. Cells in which the promoter is highly methylated are 
      auxotrophs and express ASY at very low levels. Electromobility shift assays 
      (EMSA) of nuclear extracts with oligomers from the promoting region show, in 
      addition to recognized transcription factor binding, a novel methyl binding 
      protein specific for a 12 base consensus sequence, which includes a single 
      methylated CpG. This sequence overlaps that of the amino-acid response unit of 
      the ASY promoter, which is activated byATF4 and C/EBP. Competition by the methyl 
      binding protein could account for the observed failure of the methylated promoter 
      to bind these transcription factors and consequently, although other mechanisms 
      can also be operative, for the specific repression of the gene. The ASY methyl 
      binding protein (ASMB) is present in leukemic lymphoid and myeloid cells 
      irrespective of their methylation status, and in normal lymphocytes after 
      phytohemagglutinin stimulation. It has been purified by affinity chromatography 
      and has a molecular size of 40 kDa in 10% SDS-polyacrylamide gels.
FAU - Ren, Y
AU  - Ren Y
AD  - Department of Pathology, North Shore University Hospital, Manhasset, NY 11030, 
      USA.
FAU - Roy, S
AU  - Roy S
FAU - Ding, Y
AU  - Ding Y
FAU - Iqbal, J
AU  - Iqbal J
FAU - Broome, J D
AU  - Broome JD
LA  - eng
PT  - Journal Article
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (MBD1 protein, human)
RN  - 0 (Transcription Factors)
RN  - EC 6.3.1.1 (Aspartate-Ammonia Ligase)
SB  - IM
MH  - Aspartate-Ammonia Ligase/*genetics/metabolism
MH  - Base Sequence
MH  - DNA Methylation
MH  - DNA-Binding Proteins/*metabolism
MH  - Humans
MH  - In Vitro Techniques
MH  - Leukemia/*metabolism
MH  - Molecular Sequence Data
MH  - *Promoter Regions, Genetic
MH  - Transcription Factors
MH  - Tumor Cells, Cultured
EDAT- 2004/03/30 05:00
MHDA- 2004/06/21 10:00
CRDT- 2004/03/30 05:00
PHST- 2004/03/30 05:00 [pubmed]
PHST- 2004/06/21 10:00 [medline]
PHST- 2004/03/30 05:00 [entrez]
AID - 1207498 [pii]
AID - 10.1038/sj.onc.1207498 [doi]
PST - ppublish
SO  - Oncogene. 2004 May 13;23(22):3953-61. doi: 10.1038/sj.onc.1207498.