PMID- 15048777
OWN - NLM
STAT- MEDLINE
DCOM- 20040521
LR  - 20131121
IS  - 0006-3525 (Print)
IS  - 0006-3525 (Linking)
VI  - 73
IP  - 5
DP  - 2004 Apr 5
TI  - Brownian dynamics of interactions between glyceraldehyde-3-phosphate 
      dehydrogenase (GAPDH) mutants and F-actin.
PG  - 533-41
AB  - Brownian dynamics simulations of computer models of GAPDH mutants interacting 
      with F-actin emphasized the electrostatic nature of such interactions, and 
      confirmed the importance of four previously identified lysine residues on the 
      GAPDH structure in these interactions. Mutants were GAPDH models in which one or 
      more of the previously identified lysines had been replaced with alanine. 
      Simulations showed reduced binding of these mutants to F-actin compared to 
      wild-type GAPDH. Binding was significantly reduced by mutating the four lysines; 
      the specific electrostatic interaction energy of the quadruple mutant was -7.3 
      +/- 1.0 compared to -11.4 +/- 0.5 kcal/mol for the wild enzyme. The BD 
      simulations also reaffirmed the importance of quaternary structure for GAPDH 
      binding F-actin.
CI  - 2004 Wiley Periodicals, Inc.
FAU - Waingeh, Victor F
AU  - Waingeh VF
AD  - Department of Chemistry, University of North Dakota, Grand Forks, ND 58202, USA.
FAU - Lowe, Stephen L
AU  - Lowe SL
FAU - Thomasson, Kathryn A
AU  - Thomasson KA
LA  - eng
GR  - 2R15 GM 55929-02/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biopolymers
JT  - Biopolymers
JID - 0372525
RN  - 0 (Actins)
RN  - EC 1.2.1.9 (Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+))
RN  - K3Z4F929H6 (Lysine)
SB  - IM
MH  - Actins/*chemistry
MH  - Animals
MH  - Computer Simulation
MH  - Diffusion
MH  - Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+)/*chemistry/genetics
MH  - Lysine
MH  - Models, Molecular
MH  - *Mutation
MH  - Protein Binding/genetics
MH  - Protein Structure, Quaternary
MH  - Rabbits
MH  - Static Electricity
EDAT- 2004/03/30 05:00
MHDA- 2004/05/22 05:00
CRDT- 2004/03/30 05:00
PHST- 2004/03/30 05:00 [pubmed]
PHST- 2004/05/22 05:00 [medline]
PHST- 2004/03/30 05:00 [entrez]
AID - 10.1002/bip.10560 [doi]
PST - ppublish
SO  - Biopolymers. 2004 Apr 5;73(5):533-41. doi: 10.1002/bip.10560.