PMID- 15049859
OWN - NLM
STAT- MEDLINE
DCOM- 20040615
LR  - 20131121
IS  - 0953-8194 (Print)
IS  - 0953-8194 (Linking)
VI  - 16
IP  - 3
DP  - 2004 Mar
TI  - Roles of corticotropin-releasing factor, neuropeptide Y and corticosterone in the 
      regulation of food intake in Xenopus laevis.
PG  - 279-88
AB  - In mammals, hypothalamic control of food intake involves counterregulation of 
      appetite by an orexigenic peptides such as corticotropin-releasing factor (CRF), 
      and orexigenic peptides such as neuropeptide Y (NPY). Glucocorticoids also 
      stimulate food intake by inhibiting CRF while facilitating NPY actions. To gain a 
      better understanding of the diversity and evolution of neuroendocrine feeding 
      controls in vertebrates, we analysed the effects of CRF, NPY and glucocorticoids 
      on food intake in juvenile Xenopus laevis. We also analysed brain CRF and NPY 
      mRNA content and plasma corticosterone concentrations in relation to nutritional 
      state. Intracerebroventricular (i.c.v.) injection of ovine CRF suppressed food 
      intake while CRF receptor antagonist alpha helical CRF(9-41) significantly 
      increased food intake relative to uninjected and placebo controls. By contrast, 
      i.c.v. injection of frog NPY and short-term corticosterone treatment increased 
      food intake. Semi-quantitative reverse transcription-polymerase chain reaction 
      analyses showed that CRF and NPY mRNA fluctuated with food intake in the brain 
      region containing the mid-posterior hypothalamus, pretectum, and optic tectum: 
      CRF mRNA decreased 6 h after a meal and remained low through 31 days of food 
      deprivation; NPY mRNA content also decreased 6 h after a meal, but increased to 
      prefeeding levels by 24 h. Plasma corticosterone concentration increased 6 h 
      after a meal, returned to prefeeding levels by 24 h, and did not change with 
      prolonged food deprivation. This postprandial increase in plasma corticosterone 
      may be related to the subsequent increase in plasma glucose and body water 
      content that occurs 24 h postfeeding. Overall, our data support the conclusion 
      that, similar to other vertebrates, CRF is anorexigenic while NPY is orexigenic 
      in X. laevis, and CRF secretion modulates food intake in the absence of stress by 
      exerting an inhibitory tone on appetite. Furthermore, the stress axis is 
      activated in response to food intake, but in contrast to mammals and birds is not 
      activated during periods of food deprivation.
FAU - Crespi, E J
AU  - Crespi EJ
AD  - Department of Molecular, Cellular, and Developmental Biology, University of 
      Michigan, Ann Arbor, MI 48109-1048, USA. ejcrespi@umich.edu
FAU - Vaudry, H
AU  - Vaudry H
FAU - Denver, R J
AU  - Denver RJ
LA  - eng
GR  - T32-HD07048/HD/NICHD NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neuroendocrinol
JT  - Journal of neuroendocrinology
JID - 8913461
RN  - 0 (Neuropeptide Y)
RN  - 0 (RNA, Messenger)
RN  - 9015-71-8 (Corticotropin-Releasing Hormone)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - Adaptation, Physiological
MH  - Animals
MH  - Appetite Regulation/*physiology
MH  - Corticosterone/blood/*physiology
MH  - Corticotropin-Releasing Hormone/genetics/*physiology
MH  - Feeding Behavior/physiology
MH  - Hypothalamus/physiology
MH  - Neuropeptide Y/genetics/*physiology
MH  - Postprandial Period/physiology
MH  - RNA, Messenger/analysis
MH  - Superior Colliculi/physiology
MH  - Xenopus laevis/*physiology
EDAT- 2004/03/31 05:00
MHDA- 2004/06/16 05:00
CRDT- 2004/03/31 05:00
PHST- 2004/03/31 05:00 [pubmed]
PHST- 2004/06/16 05:00 [medline]
PHST- 2004/03/31 05:00 [entrez]
AID - JNE1168 [pii]
AID - 10.1111/j.0953-8194.2004.01168.x [doi]
PST - ppublish
SO  - J Neuroendocrinol. 2004 Mar;16(3):279-88. doi: 10.1111/j.0953-8194.2004.01168.x.