PMID- 15049920
OWN - NLM
STAT- MEDLINE
DCOM- 20040618
LR  - 20131121
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 6
IP  - 5
DP  - 2004 May
TI  - Micromanipulation and further identification of FISH-labelled microcolonies of a 
      dominant denitrifying bacterium in activated sludge.
PG  - 470-9
AB  - The activated sludge process relies on the formation of strong microbial flocs. 
      The knowledge about dominant floc-forming bacteria is at present very limited, 
      especially from a phylogenetic perspective. In this study, numerous microcolonies 
      in the activated sludge flocs were found to be targeted by a 
      Betaproteobacteria-group-specific oligonucleotide probe using fluorescence in 
      situ hybridization (FISH). Some of these were micromanipulated and further 
      identified by reverse transcriptase polymerase chain reaction (RT-PCR) and 
      sequencing to belong to the Aquaspirillum genus in the Neisseriaceae family. A 
      specific oligonucleotide probe, Aqs997, was designed to target the identified 
      bacteria. A survey in nine different wastewater treatment plants with nutrient 
      removal (WWTP) showed a high abundance of bacteria hybridizing to the 
      oligonucleotide probe developed. Microautoradiography (MAR) combined with FISH on 
      activated sludge incubated with radiolabelled substrate showed uptake of 
      substrate with oxygen, nitrate and nitrite as electron acceptor demonstrating a 
      denitrifying potential of the bacteria investigated. The Aquaspirillum-related 
      bacteria seemed to be abundant denitrifiers in WWTPs with nitrogen removal and 
      they were particularly numerous in plants mainly receiving domestic wastewater, 
      where they constituted up to 30% of all bacteria.
FAU - Thomsen, T R
AU  - Thomsen TR
AD  - Department of Life Sciences, Aalborg University, Sohngaardsholmsvej 57, DK-9000 
      Aalborg, Denmark.
FAU - Nielsen, J L
AU  - Nielsen JL
FAU - Ramsing, N B
AU  - Ramsing NB
FAU - Nielsen, P H
AU  - Nielsen PH
LA  - eng
SI  - GENBANK/AY322151
SI  - GENBANK/AY322152
SI  - GENBANK/AY322153
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Bacteria/classification/*genetics/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Nitrogen/metabolism
MH  - Oligonucleotide Probes/*metabolism
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sewage/*microbiology
MH  - *Water Microbiology
MH  - Water Purification
EDAT- 2004/03/31 05:00
MHDA- 2004/06/23 05:00
CRDT- 2004/03/31 05:00
PHST- 2004/03/31 05:00 [pubmed]
PHST- 2004/06/23 05:00 [medline]
PHST- 2004/03/31 05:00 [entrez]
AID - EMI580 [pii]
AID - 10.1111/j.1462-2920.2004.00580.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2004 May;6(5):470-9. doi: 10.1111/j.1462-2920.2004.00580.x.