PMID- 1505104
OWN - NLM
STAT- MEDLINE
DCOM- 19920918
LR  - 20170112
IS  - 0392-856X (Print)
IS  - 0392-856X (Linking)
VI  - 10
IP  - 2
DP  - 1992 Mar-Apr
TI  - Interferon-gamma increases human neutrophil-mediated cartilage proteoglycan 
      degradation.
PG  - 123-9
AB  - Interferon gamma (IFN-gamma) is known to activate neutrophils and is produced by 
      the synoviocytes, macrophages, T cells and other inflammatory cells in rheumatoid 
      arthritis. Neutrophils participate in articular cartilage destruction and 
      predominate in the synovial fluid of inflamed joints during the early stages and 
      acute exacerbations of the disease. In this study we investigated the effect of 
      recombinant human IFN-gamma on human neutrophil damage to bovine articular 
      cartilage explants which had been coated with heat-aggregated IgG (HAGG). 
      Cartilage proteoglycan degradation by neutrophils was augmented by IFN-gamma at 
      10(2)-10(4) U/ml. IFN-gamma also increased neutrophil-mediated proteoglycan 
      degradation when the cells were preincubated with IFN-gamma and washed before 
      addition to cartilage. This activity of IFN-gamma was abolished by boiling and 
      was unaffected by polymixin B, indicating that the effects of IFN-gamma were not 
      due to endotoxin contamination. In the absence of neutrophils IFN-gamma had no 
      effect on proteoglycan metabolism, and the ability of IFN-gamma to increase 
      neutrophil-mediated proteoglycan degradation did not require living chondrocytes. 
      Adherence of neutrophils to HAGG-coated and uncoated cartilage explants was 
      enhanced by IFN-gamma. Opsonisation of the cartilage with HAGG also increased the 
      adhesion of neutrophils. In contrast to the enhancement of neutrophil-mediated 
      proteoglycan degradation, IFN-gamma had no effect on the inhibition of 
      proteoglycan synthesis by neutrophils.
FAU - Kowanko, I C
AU  - Kowanko IC
AD  - Department of Immunology, Adelaide Children's Hospital, Australia.
FAU - Ferrante, A
AU  - Ferrante A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Clin Exp Rheumatol
JT  - Clinical and experimental rheumatology
JID - 8308521
RN  - 0 (Endotoxins)
RN  - 0 (Proteoglycans)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Cartilage, Articular/*metabolism/pathology/physiology
MH  - Cattle
MH  - Cell Adhesion/drug effects/physiology
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - Endotoxins/pharmacology
MH  - Humans
MH  - Interferon-gamma/*pharmacology
MH  - Neutrophils/*physiology
MH  - Proteoglycans/*metabolism
EDAT- 1992/03/01 00:00
MHDA- 1992/03/01 00:01
CRDT- 1992/03/01 00:00
PHST- 1992/03/01 00:00 [pubmed]
PHST- 1992/03/01 00:01 [medline]
PHST- 1992/03/01 00:00 [entrez]
PST - ppublish
SO  - Clin Exp Rheumatol. 1992 Mar-Apr;10(2):123-9.