PMID- 15051157
OWN - NLM
STAT- MEDLINE
DCOM- 20040603
LR  - 20061115
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 125
IP  - 1
DP  - 2004
TI  - Fate of autologous dermal stem cells transplanted into the spinal cord after 
      traumatic injury (TSCI).
PG  - 179-89
AB  - Rat dermis is a source of cells capable of growing in vitro and, in appropriate 
      conditions, forming floating spheres constituted by nestin-positive cells. We 
      have clonally grown these spheres up to the 15th generation. These spheres can be 
      dissociated into cells that differentiate in vitro under appropriate conditions, 
      these cells are labeled by antibodies to immature neuron markers such as nestin 
      and beta-tubulin III and, later, to mature neuron markers such as 
      microtubule-associated protein 2 and neurofilaments. However, most cells are 
      positive to the astroglial marker glia fibrillary acidic protein (GFAP). When 
      sphere-derived cells are transplanted into the spinal cord after traumatic 
      injury, their migration into the lesion cavity is optimal but their 
      differentiation is dependent upon the time interval between lesioning and cell 
      transplantation. Injection of skin-derived stem cell within 30 min from injury 
      yields mainly membrane activated complex-1 (MAC-1), cluster of differentiation-4 
      (CD-4) and CD-8 positive cells, that 60-90 days later undergo apoptosis. However, 
      when transplantation is performed 7 days after injury, most cells (65% of total) 
      are positive to staining with antibodies to GFAP, others (16%) to neurofilaments, 
      and a smaller amount (2%) to the endothelial marker, platelet endothelial cell 
      adhesion molecule. Thus our study shows that delayed transplantations of 
      dermis-derived stem cells yield healthy cells that do not die, migrate to the 
      lesion site, and there differentiate mainly in cells expressing glia and neuronal 
      markers. On the other hand there is the possibility of dye transfer from labeled 
      cells to endogenous cells, and this might influence the data.
FAU - Gorio, A
AU  - Gorio A
AD  - Laboratory of Pharmacology, Department of Medicine, Surgery and Dentistry, 
      Faculty of Medicine, University of Milan, Via A di Rudini 8, Milano 20142, Italy. 
      alfredo.gorio@unimi.it
FAU - Torrente, Y
AU  - Torrente Y
FAU - Madaschi, L
AU  - Madaschi L
FAU - Di Stefano, A B
AU  - Di Stefano AB
FAU - Pisati, F
AU  - Pisati F
FAU - Marchesi, C
AU  - Marchesi C
FAU - Belicchi, M
AU  - Belicchi M
FAU - Di Giulio, A M
AU  - Di Giulio AM
FAU - Bresolin, N
AU  - Bresolin N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Glial Fibrillary Acidic Protein)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cell Differentiation/*physiology
MH  - Cell Movement/physiology
MH  - Dermis/*cytology/metabolism
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Male
MH  - Neurons/*physiology
MH  - Polymerase Chain Reaction
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Recovery of Function/physiology
MH  - Spinal Cord Injuries/*therapy
MH  - *Stem Cell Transplantation
MH  - Stem Cells/cytology/metabolism
MH  - Time Factors
EDAT- 2004/03/31 05:00
MHDA- 2004/06/04 05:00
CRDT- 2004/03/31 05:00
PHST- 2003/10/24 00:00 [accepted]
PHST- 2004/03/31 05:00 [pubmed]
PHST- 2004/06/04 05:00 [medline]
PHST- 2004/03/31 05:00 [entrez]
AID - S0306452203008315 [pii]
AID - 10.1016/j.neuroscience.2003.10.046 [doi]
PST - ppublish
SO  - Neuroscience. 2004;125(1):179-89. doi: 10.1016/j.neuroscience.2003.10.046.