PMID- 15051240
OWN - NLM
STAT- MEDLINE
DCOM- 20041005
LR  - 20161124
IS  - 0160-4120 (Print)
IS  - 0160-4120 (Linking)
VI  - 30
IP  - 5
DP  - 2004 Jul
TI  - Waste wood recycling as animal bedding and development of bio-monitoring tool 
      using the CALUX assay.
PG  - 639-49
AB  - Animal bedding made of waste wood samples from seven different plants in Japan 
      were chemically analyzed in terms of persistent organic pollutants (POPs) 
      including polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/DFs), 
      coplanar polychlorinated biphenyls (Co-PCBs), drin compounds, chlordane compounds 
      and various inorganic toxic compounds (Cr, Cu, As, B, Cd and Pb) to investigate 
      the chemical characteristics and levels of contamination. Further investigation 
      was conducted to determine the success of applying the Chemically Activated 
      Luciferase Expression (CALUX) bioassay to the waste wood samples in combination 
      with a cleanup procedure for the detection of dioxin-like compounds in order to 
      develop the CALUX bioassay as a rapid and cost-effective screening/monitoring 
      method and a contributive tool to risk management in the waste wood recycling 
      process. For the cleanup procedure, crude extracts from wood samples were 
      prepared by dimethylsulfoxide (DMSO)/n-hexane extraction, and then the extracts 
      were processed by silica gel-44% sulfuric acid reflux treatment at 70 degrees C 
      for 60 min to yield the bioassay fractions. The presence of POPs and inorganic 
      toxic compounds were confirmed in most of the litter samples. In particular, 
      Co-PCBs in one sample (litter dust) showed a high concentration level (1200000 
      pg/g, 240 pg TEQ/g), suggesting the potential for contamination from demolition 
      waste. The CALUX assay-determined TEQs (CALUX-TEQs) were significantly high in 
      the sample after DMSO/n-hexane extraction, probably due to labile aryl 
      hydrocarbon receptor (AhR) ligands such as PAHs; however, they were remarkably 
      reduced through a single silica gel-44% sulfuric acid reflux treatment. The ratio 
      between CALUX-TEQ values and WHO toxicity equivalent values (WHO-TEQ) obtained by 
      congener-specific chemical analysis ranged from 0.058 to 22 and show 
      comparatively good agreement. Underestimation in some samples, however, was 
      observed where WHO-TEQ values of Co-PCBs contributed greatly to total WHO-TEQ 
      values. Reasons for this gap could be lower CALUX assay-determined relative 
      potencies (REPs) than the WHO-TEFs for these congeners or AhR-antagonistic 
      effects of non dioxin-like PCBs which coexist at higher concentration than 
      Co-PCBs. The CALUX assay is proposed as a promising application in the recycling 
      process of wooden materials.
FAU - Asari, Misuzu
AU  - Asari M
AD  - Environment Preservation Center, Kyoto University, Kyoto 606-8501, Japan. 
      asari@student.eprc.kyoto-u.ac.jp
FAU - Takatsuki, Hiroshi
AU  - Takatsuki H
FAU - Yamazaki, Michifumi
AU  - Yamazaki M
FAU - Azuma, Tomonori
AU  - Azuma T
FAU - Takigami, Hidetaka
AU  - Takigami H
FAU - Sakai, Shin-Ichi
AU  - Sakai S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Environ Int
JT  - Environment international
JID - 7807270
RN  - 0 (Benzofurans)
RN  - 0 (Dibenzofurans, Polychlorinated)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Polychlorinated Dibenzodioxins)
RN  - 0 (Soil Pollutants)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Animals
MH  - Benzofurans/*analysis
MH  - Biological Assay
MH  - Carcinoma, Hepatocellular/pathology
MH  - *Conservation of Natural Resources
MH  - Dibenzofurans, Polychlorinated
MH  - Environmental Monitoring/methods
MH  - Environmental Pollutants/*analysis
MH  - *Housing, Animal
MH  - Japan
MH  - Liver Neoplasms/pathology
MH  - Luciferases/analysis/biosynthesis
MH  - Polychlorinated Biphenyls/*analysis
MH  - Polychlorinated Dibenzodioxins/*analogs & derivatives/*analysis
MH  - Rats
MH  - Soil Pollutants/*analysis
MH  - Tumor Cells, Cultured
MH  - *Wood
EDAT- 2004/03/31 05:00
MHDA- 2004/10/06 09:00
CRDT- 2004/03/31 05:00
PHST- 2003/10/02 00:00 [received]
PHST- 2003/12/03 00:00 [accepted]
PHST- 2004/03/31 05:00 [pubmed]
PHST- 2004/10/06 09:00 [medline]
PHST- 2004/03/31 05:00 [entrez]
AID - S0160412003002484 [pii]
AID - 10.1016/j.envint.2003.12.002 [doi]
PST - ppublish
SO  - Environ Int. 2004 Jul;30(5):639-49. doi: 10.1016/j.envint.2003.12.002.