PMID- 15051392
OWN - NLM
STAT- MEDLINE
DCOM- 20040517
LR  - 20061115
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 321
IP  - 2
DP  - 2004 Apr 10
TI  - Heparan sulfate proteoglycan mediates the selective attachment and 
      internalization of serotype 3 human adenovirus dodecahedron.
PG  - 332-40
AB  - During adenovirus type 3 (Ad3) infection cycle, the penton (Pt) of the viral 
      capsid, a noncovalent complex of fiber and penton base proteins, is produced in 
      large excess and self-assembles to form a highly organized dodecahedral 
      structure, termed dodecahedron (Dd). The physiological role of these particles is 
      poorly understood, but we have recently reported that they can penetrate cells 
      with high efficiency and thus may constitute an attractive tool for gene or 
      protein delivery approaches. Surprisingly, Dd displayed the ability to enter 
      cells non-permissive to Ad3, suggesting the existence of additional 
      internalization modes. In this study, we show that Ad3 Dd binds to cell surface 
      heparan sulfate (HS) through high affinity interaction with the penton base. 
      Furthermore, binding to HS was found to be the prerequisite for a novel and Dd 
      specific entry pathway that could not be used by Ad3. Overall, these data provide 
      new insights in the possible role of Dd during viral infection and potential 
      therapeutic applications.
FAU - Vives, Romain R
AU  - Vives RR
AD  - Institut de Biologie Structurale, CNRS-CEA-UJF, 38027 Grenoble, France.
FAU - Lortat-Jacob, Hugues
AU  - Lortat-Jacob H
FAU - Chroboczek, Jadwiga
AU  - Chroboczek J
FAU - Fender, Pascal
AU  - Fender P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Antigens, Viral)
RN  - 0 (Capsid Proteins)
RN  - 0 (Heparan Sulfate Proteoglycans)
RN  - 0 (Receptors, Virus)
RN  - 0 (hexon capsid protein, Adenovirus)
RN  - 0 (penton protein, adenovirus)
SB  - IM
MH  - Adenoviruses, Human/chemistry/*metabolism
MH  - Animals
MH  - Antigens, Viral/*metabolism
MH  - Capsid Proteins/*metabolism
MH  - Cell Line
MH  - Cricetinae
MH  - HeLa Cells
MH  - Heparan Sulfate Proteoglycans/*metabolism
MH  - Humans
MH  - Receptors, Virus/*metabolism
MH  - Virus Replication
EDAT- 2004/03/31 05:00
MHDA- 2004/05/18 05:00
CRDT- 2004/03/31 05:00
PHST- 2003/11/19 00:00 [received]
PHST- 2004/01/09 00:00 [revised]
PHST- 2004/01/09 00:00 [accepted]
PHST- 2004/03/31 05:00 [pubmed]
PHST- 2004/05/18 05:00 [medline]
PHST- 2004/03/31 05:00 [entrez]
AID - S0042682204000583 [pii]
AID - 10.1016/j.virol.2004.01.015 [doi]
PST - ppublish
SO  - Virology. 2004 Apr 10;321(2):332-40. doi: 10.1016/j.virol.2004.01.015.