PMID- 15051397
OWN - NLM
STAT- MEDLINE
DCOM- 20040517
LR  - 20061115
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 321
IP  - 2
DP  - 2004 Apr 10
TI  - Genomic characterization of virulent, attenuated, and revertant passages of a 
      North American porcine reproductive and respiratory syndrome virus strain.
PG  - 383-90
AB  - Pigs were exposed to three passages of the NADC-8 strain of porcine reproductive 
      and respiratory syndrome virus (PRRSV) to investigate the relationship between 
      genotypic and phenotypic properties. Differences were found in the virulence of 
      the three passages called virulent, intermediate, and avirulent. Avirulent virus 
      was derived by attenuation of virulent virus in cell culture and intermediate 
      virus was derived by passage of avirulent virus in a pig. Nucleotide sequence 
      differences between virulent and avirulent virus consisted of 50 nucleotide 
      changes and a three-nucleotide deletion, and between avirulent and intermediate 
      virus consisted of 8 nucleotide changes resulting in six amino acid changes. 
      Three of these amino acid changes were direct reversions to virulent virus. 
      Genetic changes, especially those seemingly associated with attenuation followed 
      by some degree of reversion to virulence, in ORF1a, ORF1b, and ORF 6 regions of 
      the genome may be involved in the control of PRRSV replication and virulence.
FAU - Grebennikova, T V
AU  - Grebennikova TV
AD  - NARVAC R and D Department, Ivanovski Virology Institute, 123098 Moscow, Russia. 
      grebennikova@narvac.com
FAU - Clouser, D F
AU  - Clouser DF
FAU - Vorwald, A C
AU  - Vorwald AC
FAU - Musienko, M I
AU  - Musienko MI
FAU - Mengeling, W L
AU  - Mengeling WL
FAU - Lager, K M
AU  - Lager KM
FAU - Wesley, R D
AU  - Wesley RD
FAU - Biketov, S F
AU  - Biketov SF
FAU - Zaberezhny, A D
AU  - Zaberezhny AD
FAU - Aliper, T I
AU  - Aliper TI
FAU - Nepoklonov, E A
AU  - Nepoklonov EA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Amino Acids)
RN  - 0 (Nucleotides)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Amino Acids/metabolism
MH  - Animals
MH  - Disease Models, Animal
MH  - Genome, Viral
MH  - Mutation
MH  - Nucleotides/metabolism
MH  - Open Reading Frames
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/pathogenicity
MH  - Swine
MH  - Viral Proteins/genetics/metabolism
MH  - Virulence/*genetics
EDAT- 2004/03/31 05:00
MHDA- 2004/05/18 05:00
CRDT- 2004/03/31 05:00
PHST- 2003/09/15 00:00 [received]
PHST- 2003/12/20 00:00 [revised]
PHST- 2004/01/04 00:00 [accepted]
PHST- 2004/03/31 05:00 [pubmed]
PHST- 2004/05/18 05:00 [medline]
PHST- 2004/03/31 05:00 [entrez]
AID - S0042682204000200 [pii]
AID - 10.1016/j.virol.2004.01.001 [doi]
PST - ppublish
SO  - Virology. 2004 Apr 10;321(2):383-90. doi: 10.1016/j.virol.2004.01.001.