PMID- 15053136 OWN - NLM STAT- MEDLINE DCOM- 20040507 LR - 20190702 IS - 0042-4900 (Print) IS - 0042-4900 (Linking) VI - 154 IP - 10 DP - 2004 Mar 6 TI - Attempts to transmit porcine reproductive and respiratory syndrome virus by aerosols under controlled field conditions. PG - 294-7 AB - An experimental infection with porcine reproductive and respiratory syndrome virus (PRRSV) was established in 150 five-month-old pigs housed in a fan-ventilated finishing facility, the infected barn. To determine whether air exhausted from the wall fans contained infectious PRRSV, a trailer containing 10 four-week-old PRRSV-naive sentinel pigs was placed 10 m from the building from day 3 after the 150 pigs were infected until day 10. To connect the two airspaces, one end of an opaque plastic tube, 15 m in length and 5 cm in diameter, was fastened to the wall fan of the infected barn, and the other end was placed inside the trailer. Air from the building was exhausted into the trailer 24 hours a day for seven consecutive days and PRRSV infection was monitored in the infected pigs and the sentinel pigs. Air samples were collected from the infected barn and the trailer. PRRSV infection was detected in the infected pigs three and seven days after they were infected, but not in the sentinel pigs. All the air samples were negative for PRRSV by PCR, virus isolation and a pig bioassay. FAU - Trincado, C AU - Trincado C AD - University of Minnesota, Swine Disease Eradication Center, 385C Animal Science/Veterinary Medicine Building, 1988 Fitch Avenue, St Paul, MN 55108, USA. FAU - Dee, S AU - Dee S FAU - Jacobson, L AU - Jacobson L FAU - Otake, S AU - Otake S FAU - Rossow, K AU - Rossow K FAU - Pijoan, C AU - Pijoan C LA - eng PT - Journal Article PL - England TA - Vet Rec JT - The Veterinary record JID - 0031164 RN - 0 (Aerosols) SB - IM MH - *Aerosols MH - *Animal Husbandry MH - Animals MH - Disease Transmission, Infectious/*veterinary MH - Porcine Reproductive and Respiratory Syndrome/*transmission MH - Porcine respiratory and reproductive syndrome virus/*pathogenicity MH - Swine EDAT- 2004/04/01 05:00 MHDA- 2004/05/08 05:00 CRDT- 2004/04/01 05:00 PHST- 2004/04/01 05:00 [pubmed] PHST- 2004/05/08 05:00 [medline] PHST- 2004/04/01 05:00 [entrez] AID - 10.1136/vr.154.10.294 [doi] PST - ppublish SO - Vet Rec. 2004 Mar 6;154(10):294-7. doi: 10.1136/vr.154.10.294.