PMID- 15053933
OWN - NLM
STAT- MEDLINE
DCOM- 20041116
LR  - 20131121
IS  - 0021-9975 (Print)
IS  - 0021-9975 (Linking)
VI  - 130
IP  - 4
DP  - 2004 May
TI  - Expression of interferon-alpha and Mx1 protein in pigs acutely infected with 
      porcine reproductive and respiratory syndrome virus (PRRSV).
PG  - 299-305
AB  - The expression of mRNA encoding interferon-alpha (IFN-alpha) and Mx1 protein was 
      studied, by reverse transcription-polymerase chain reaction and by in-situ 
      hybridization with a non-radioactive digoxigenin-labelled cDNA probe, in 
      formalin-fixed, paraffin wax-embedded lung tissue from pigs experimentally 
      infected with a Korean isolate (North American genotype) of porcine reproductive 
      and respiratory syndrome virus (PRRSV). The animals were examined over a period 
      of 10 days after intranasal inoculation. IFN-alpha and Mx1 protein was detected 
      in the lung at 1 day post inoculation (dpi), the number of positive cells 
      increasing at 7dpi, and rapidly decreasing thereafter. Hybridization signals for 
      IFN-alpha and Mx1 protein were usually associated with inflammation, and in 
      particular with macrophages. Expression of IFN-alpha and Mx1 protein was negative 
      in non-lesional lung of PRRSV-infected pigs and in normal lung from control pigs. 
      There was a good statistical correlation between the number of cells positive for 
      mRNA encoding IFN-alpha and Mx1 protein in the infected lungs (r = 0.95, P< 
      0.05). The results suggest that the expression of IFN-alpha and Mx1 protein plays 
      a role in the early host defence against PRRSV infection.
FAU - Chung, H-K
AU  - Chung HK
AD  - Department of Veterinary Pathology, College of Veterinary Medicine and School of 
      Agricultural Biotechnology, Seoul National University, San 56-1, Kwanak-Gu, Seoul 
      151-742, South Korea.
FAU - Lee, J-H
AU  - Lee JH
FAU - Kim, S-H
AU  - Kim SH
FAU - Chae, C
AU  - Chae C
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Comp Pathol
JT  - Journal of comparative pathology
JID - 0102444
RN  - 0 (Interferon-alpha)
RN  - 0 (Myxovirus Resistance Proteins)
RN  - 0 (RNA, Messenger)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Animals
MH  - GTP-Binding Proteins/*biosynthesis
MH  - In Situ Hybridization
MH  - Interferon-alpha/*biosynthesis
MH  - Lung/*metabolism/pathology
MH  - Macrophages/metabolism
MH  - Myxovirus Resistance Proteins
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/*metabolism
MH  - Porcine respiratory and reproductive syndrome virus/immunology
MH  - RNA, Messenger/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Swine
MH  - Swine Diseases/immunology/*metabolism
EDAT- 2004/04/01 05:00
MHDA- 2004/11/17 09:00
CRDT- 2004/04/01 05:00
PHST- 2003/06/10 00:00 [received]
PHST- 2003/12/09 00:00 [accepted]
PHST- 2004/04/01 05:00 [pubmed]
PHST- 2004/11/17 09:00 [medline]
PHST- 2004/04/01 05:00 [entrez]
AID - S0021997503001427 [pii]
AID - 10.1016/j.jcpa.2003.12.006 [doi]
PST - ppublish
SO  - J Comp Pathol. 2004 May;130(4):299-305. doi: 10.1016/j.jcpa.2003.12.006.