PMID- 15054677
OWN - NLM
STAT- MEDLINE
DCOM- 20041109
LR  - 20181113
IS  - 0095-3628 (Print)
IS  - 0095-3628 (Linking)
VI  - 47
IP  - 3
DP  - 2004 Apr
TI  - Distribution of Nevskia ramosa and other rosette-forming neustonic bacteria.
PG  - 218-23
AB  - Samples from 27 natural and artificial aquatic environments were analyzed for the 
      presence of rosette-forming bacteria by a combined cultivation and molecular 
      biological approach. Rosette-forming bacteria developed in 20 enrichment cultures 
      with ammonia-free medium under air. Three morphotypes could be distinguished. The 
      most abundant type I resembled Nevskia ramosa and formed hydrophobic, flat, and 
      dichotomously branching rosettes. Type II rosettes were three-dimensional and 
      were observed in 10 enrichments, often together with those of type I. These 
      rosettes were hydrophilic indicating life in the hyponeuston underneath the 
      air-water interface. Rosettes of a third type consisted of hydrophilic slime 
      stalks that were excreted at the cell poles and were observed in only one sample. 
      Using fluorescence in situ hybridization (FISH) with the Nevskia-specific probes 
      NEV177 and NEV656, the presence of Nevskia ramosa was demonstrated in exactly 
      those samples that showed type I rosettes. In a series of most-probable-number 
      experiments, during a calm and sunny weather period 430,000 Nevskia-like bacteria 
      per mL were found in surface samples, while during rainy weather and within the 
      water body the numbers were lower by several orders of magnitude. Five pure 
      cultures isolated from various enrichments were characterized in detail. The two 
      isolates forming type I rosettes were identified as Nevskia ramosa by 16S rDNA 
      analysis. However, comparison by genomic fingerprinting (ERIC-PCR) revealed 
      differences between the two isolates and previously characterized strains. The 
      16S rDNA of two isolates forming type II rosettes showed 97.6% similarity to that 
      of Pseudomonas fluorescens. The closest relative of the isolate forming type III 
      rosettes was Sphingomonas parapaucimobilis (96.4% sequence similarity of the 16S 
      rRNA sequence). All isolates grew homogeneously submersed if ammonia was added to 
      the medium. Our results indicate that Nevskia ramosa is a widely distributed 
      epineustonic bacterium, which can specifically be deleted by its flat and 
      hydrophobic rosettes on ammonia-free media.
FAU - Pladdies, Teresa
AU  - Pladdies T
AD  - Institut fur Chemie und Biologie des Meeres, Universitat Oldenburg, D-26111, 
      Oldenburg, Germany.
FAU - Babenzien, Hans-Dietrich
AU  - Babenzien HD
FAU - Cypionka, Heribert
AU  - Cypionka H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20040402
PL  - United States
TA  - Microb Ecol
JT  - Microbial ecology
JID - 7500663
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - *Biofilms
MH  - DNA Fingerprinting
MH  - Demography
MH  - Europe
MH  - In Situ Hybridization, Fluorescence
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Sequence Homology
MH  - Species Specificity
MH  - *Water Microbiology
MH  - Weather
MH  - Xanthomonadaceae/*cytology/*genetics
EDAT- 2004/04/01 05:00
MHDA- 2004/11/13 09:00
CRDT- 2004/04/01 05:00
PHST- 2003/02/09 00:00 [received]
PHST- 2003/04/12 00:00 [accepted]
PHST- 2004/04/01 05:00 [pubmed]
PHST- 2004/11/13 09:00 [medline]
PHST- 2004/04/01 05:00 [entrez]
AID - 10.1007/s00248-003-1070-3 [doi]
PST - ppublish
SO  - Microb Ecol. 2004 Apr;47(3):218-23. doi: 10.1007/s00248-003-1070-3. Epub 2004 Apr 
      2.