PMID- 15056227
OWN - NLM
STAT- MEDLINE
DCOM- 20040420
LR  - 20111117
IS  - 0903-4641 (Print)
IS  - 0903-4641 (Linking)
VI  - 112
IP  - 2
DP  - 2004 Feb
TI  - Staphylococcus aureus, but not Staphylococcus epidermidis, modulates the 
      oxidative response and induces apoptosis in human neutrophils.
PG  - 109-18
AB  - S. epidermidis is the most common isolate in foreign body infections. The aim of 
      this study was to understand why S. epidermidis causes silent biomaterial 
      infections. In view of the divergent inflammatory responses S. epidermidis and S. 
      aureus cause in patients, we analyzed how they differ when interacting with human 
      neutrophils. Neutrophils interacting with S. epidermidis strains isolated either 
      from granulation tissue covering infected hip prostheses or from normal skin 
      flora were tested by measuring the oxidative response as chemiluminescence and 
      apoptosis as annexin V binding. Different S. aureus strains were tested in 
      parallel. All S. epidermidis tested were unable to modulate the oxidative 
      reaction in response to formyl-methionyl-leucyl-phenylalanine (fMLP) and did not 
      provoke, but rather inhibited, apoptosis. In contrast, some S. aureus strains 
      enhanced the oxidative reaction, and this priming capacity was linked to 
      p38-mitogen-activated-protein-kinase (p38-MAPK) activation and induction of 
      apoptosis. Our results may explain why S. epidermidis is a weak inducer of 
      inflammation compared to S. aureus, and therefore responsible for the indolent 
      and chronic course of S. epidermidis biomaterial infections.
FAU - Nilsdotter-Augustinsson, Asa
AU  - Nilsdotter-Augustinsson A
AD  - Division of Infectious Diseases, Department of Molecular and Clinical Medicine, 
      Linkoping University, Linkoping, Sweden. asa.nilsdotter@lio.se
FAU - Wilsson, Asa
AU  - Wilsson A
FAU - Larsson, Jenny
AU  - Larsson J
FAU - Stendahl, Olle
AU  - Stendahl O
FAU - Ohman, Lena
AU  - Ohman L
FAU - Lundqvist-Gustafsson, Helen
AU  - Lundqvist-Gustafsson H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - APMIS
JT  - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
JID - 8803400
RN  - 0 (Annexin A5)
RN  - 0 (Biocompatible Materials)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Annexin A5/metabolism
MH  - Apoptosis/*immunology
MH  - Biocompatible Materials
MH  - Blotting, Western
MH  - Humans
MH  - Luminescent Measurements
MH  - Mitogen-Activated Protein Kinases/immunology
MH  - Neutrophils/immunology/metabolism/*microbiology
MH  - Phagocytosis/immunology
MH  - Prosthesis-Related Infections/microbiology
MH  - Respiratory Burst/*immunology
MH  - Staphylococcal Infections/immunology/*microbiology
MH  - Staphylococcus aureus/immunology/*pathogenicity
MH  - Staphylococcus epidermidis/immunology/*pathogenicity
MH  - Statistics, Nonparametric
MH  - p38 Mitogen-Activated Protein Kinases
EDAT- 2004/04/02 05:00
MHDA- 2004/04/21 05:00
CRDT- 2004/04/02 05:00
PHST- 2004/04/02 05:00 [pubmed]
PHST- 2004/04/21 05:00 [medline]
PHST- 2004/04/02 05:00 [entrez]
AID - APMapm1120205 [pii]
AID - 10.1111/j.1600-0463.2004.apm1120205.x [doi]
PST - ppublish
SO  - APMIS. 2004 Feb;112(2):109-18. doi: 10.1111/j.1600-0463.2004.apm1120205.x.