PMID- 15056898
OWN - NLM
STAT- MEDLINE
DCOM- 20041207
LR  - 20191210
IS  - 0916-8451 (Print)
IS  - 0916-8451 (Linking)
VI  - 68
IP  - 3
DP  - 2004 Mar
TI  - A novel NADH-dependent carbonyl reductase from Kluyveromyces aestuarii and 
      comparison of NADH-regeneration system for the synthesis of ethyl 
      (S)-4-chloro-3-hydroxybutanoate.
PG  - 638-49
AB  - To compare NADH-regeneration systems for the synthesis of 
      (S)-4-chloro-3-hydroxybutanoate (ECHB), a novel NADH-dependent carbonyl reductase 
      (KaCR1), which reduced ethyl 4-chloroacetoacetate (ECAA) to form (S)-ECHB, was 
      screened and purified from Kluyveromyces aestuarii and a gene encoding KaCR1 was 
      cloned. Glucose dehydrogenase (GDH) and formate dehydrogenase (FDH) were compared 
      as enzymes for NADH regeneration using Escherichia coli cells coexpressing each 
      enzyme with KaCR1. E. coli cells coexpressing GDH produced 45.6 g/l of (S)-ECHB 
      from 50 g/l of ECAA and E. coli cells coexpressing FDH, alternatively, produced 
      only 19.0 g/l. The low productivity in the case of FDH was suggested to result 
      from the low activity and instability of FDH.
FAU - Yamamoto, Hiroaki
AU  - Yamamoto H
AD  - Life Science Development Center, CPI Company, Daicel Chemical Industries, Ltd., 
      Tsukuba Research Center, Ibaraki, Japan. h-yamamoto@daicel.co.jp
FAU - Mitsuhashi, Kazuya
AU  - Mitsuhashi K
FAU - Kimoto, Norihiro
AU  - Kimoto N
FAU - Matsuyama, Akinobu
AU  - Matsuyama A
FAU - Esaki, Nobuyoshi
AU  - Esaki N
FAU - Kobayashi, Yoshinori
AU  - Kobayashi Y
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Biosci Biotechnol Biochem
JT  - Bioscience, biotechnology, and biochemistry
JID - 9205717
RN  - 0 (Acetoacetates)
RN  - 0 (Butyrates)
RN  - 0 (ethyl 4-chloro-3-hydroxybutanoate)
RN  - 0U46U6E8UK (NAD)
RN  - 5AE82250CM (ethyl 4-chloro-3-oxobutanoate)
RN  - EC 1.1.- (Alcohol Oxidoreductases)
RN  - EC 1.1.1.47 (Glucose 1-Dehydrogenase)
RN  - EC 1.17.1.9 (Formate Dehydrogenases)
RN  - EC 1.2.2.1 (Formate dehydrogenase (cytochrome))
RN  - EC 1.3.1.- (short chain trans-2-enoyl-CoA reductase)
RN  - EC 1.6.- (NADH, NADPH Oxidoreductases)
RN  - EC 2.3.1.85 (Fatty Acid Synthases)
SB  - IM
MH  - Acetoacetates/*metabolism
MH  - Alcohol Oxidoreductases/genetics/isolation & purification/*metabolism
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Butyrates/*metabolism
MH  - Cloning, Molecular
MH  - Conserved Sequence/genetics
MH  - Escherichia coli/genetics/metabolism
MH  - Fatty Acid Synthases/genetics/metabolism
MH  - Formate Dehydrogenases/metabolism
MH  - Glucose 1-Dehydrogenase/metabolism
MH  - Kluyveromyces/*enzymology
MH  - Molecular Sequence Data
MH  - NAD/*metabolism
MH  - NADH, NADPH Oxidoreductases/genetics/metabolism
MH  - Plasmids/genetics
MH  - Sequence Analysis, DNA
MH  - Substrate Specificity
EDAT- 2004/04/02 05:00
MHDA- 2004/12/16 09:00
CRDT- 2004/04/02 05:00
PHST- 2004/04/02 05:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/04/02 05:00 [entrez]
AID - 10.1271/bbb.68.638 [doi]
PST - ppublish
SO  - Biosci Biotechnol Biochem. 2004 Mar;68(3):638-49. doi: 10.1271/bbb.68.638.