PMID- 15057919 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20040426 LR - 20040525 IS - 0270-9139 (Print) IS - 0270-9139 (Linking) VI - 39 IP - 4 DP - 2004 Apr TI - An uncomfortable silence em leader while we all search for a better reporter gene in adult stem cell biology. PG - 1143-6 AB - The plasticity of bone marrow cells (BMCs) remains controversial. The present study found that persistent injury induces efficient trans-differentiation of BMCs into functional hepatocytes. Mice with liver cirrhosis induced by carbon tetrachloride were injected with 1 x 10(5) non-treated green fluorescent protein (GFP)-positive BMCs via the tail vein. In these mice, transplanted GFP-positive BMCs efficiently migrated into the peri-portal area of liver lobules after one day, repopulating 25% of the recipient liver by 4 weeks. In contrast, no GFP-positive BMCs were detected following transplantation into control mice with undamaged livers. BMCs trans-differentiated into functional mature hepatocytes via immature hepatoblasts. Serum albumin levels were significantly elevated to compensate for chronic liver failure in BMC transplantation. These results reveal that recipient conditions and microenvironments represent key factors for successful cell therapy using BMCs. FAU - McTaggart, Ryan A AU - McTaggart RA AD - UCSF Liver Center and Department of Surgery, Division of Transplantation, University of California, San Francisco, San Francisco, CA, USA. FAU - Feng, Sandy AU - Feng S LA - eng PT - Comment PT - Journal Article PL - United States TA - Hepatology JT - Hepatology (Baltimore, Md.) JID - 8302946 CON - J Biochem. 2003 Oct;134(4):551-8. PMID: 14607982 EDAT- 2004/04/02 05:00 MHDA- 2004/04/02 05:01 CRDT- 2004/04/02 05:00 PHST- 2004/04/02 05:00 [pubmed] PHST- 2004/04/02 05:01 [medline] PHST- 2004/04/02 05:00 [entrez] AID - 10.1002/hep.20192 [doi] PST - ppublish SO - Hepatology. 2004 Apr;39(4):1143-6. doi: 10.1002/hep.20192.