PMID- 15059097
OWN - NLM
STAT- MEDLINE
DCOM- 20040916
LR  - 20161124
IS  - 0105-1873 (Print)
IS  - 0105-1873 (Linking)
VI  - 50
IP  - 1
DP  - 2004 Jan
TI  - Reproducible prediction of contact allergenic potency using the local lymph node 
      assay.
PG  - 15-7
AB  - A considerable body of data has been accumulated which demonstrates that the 
      local lymph node assay (LLNA) can provide a valuable estimation of the contact 
      allergenic potency of a substance. This estimate is obtained via interpolation of 
      the LLNA dose-response curve and is expressed as the concentration of the 
      chemical required to evince a 3-fold stimulation of proliferation in lymph nodes 
      draining the site of application compared to the vehicle-treated controls (EC3). 
      It has also been shown that the EC3 estimates are reproducible and are stable 
      over time. In the present work, we have extended this information by a 
      demonstration of the inherent biological variability surrounding EC3 estimation, 
      using data derived (from a single laboratory) from the testing of isoeugenol as a 
      positive control. Isoeugenol gave EC3 values ranging from 0.5 to 2.6% (n = 29), 
      with a mean and standard deviation of 1.2 +/- 0.6%. Given that EC3 values for a 
      variety of contact allergens range over several orders of magnitude, these 
      results further endorse the utility of EC3 values as a reliable indicator of 
      human contact allergenic potency.
FAU - Basketter, David A
AU  - Basketter DA
AD  - Safety and Environmental Assurance Centre, Unilever Colworth, Sharnbrook, 
      Bedford, UK. david.basketter@unilever.com
FAU - Cadby, Peter
AU  - Cadby P
LA  - eng
PT  - Journal Article
PL  - England
TA  - Contact Dermatitis
JT  - Contact dermatitis
JID - 7604950
RN  - 0 (Allergens)
RN  - 3T8H1794QW (Eugenol)
RN  - 5M0MWY797U (isoeugenol)
SB  - IM
MH  - Administration, Topical
MH  - Allergens/administration & dosage/*adverse effects
MH  - Animals
MH  - Dermatitis, Allergic Contact/*diagnosis/etiology
MH  - Eugenol/administration & dosage/*adverse effects/*analogs & derivatives
MH  - Female
MH  - *Local Lymph Node Assay
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Predictive Value of Tests
MH  - Reproducibility of Results
EDAT- 2004/04/03 05:00
MHDA- 2004/09/17 05:00
CRDT- 2004/04/03 05:00
PHST- 2004/04/03 05:00 [pubmed]
PHST- 2004/09/17 05:00 [medline]
PHST- 2004/04/03 05:00 [entrez]
AID - COD278 [pii]
AID - 10.1111/j.0105-1873.2004.00278.x [doi]
PST - ppublish
SO  - Contact Dermatitis. 2004 Jan;50(1):15-7. doi: 10.1111/j.0105-1873.2004.00278.x.